Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues

doi:10.1114/1.1467923

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	Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues
	Zhu C(朱承); Long M(龙勉); Chesla SE; Bongrand P; Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
发表期刊	Annals of Biomedical Engineering
	2002
卷号	30 期号:3 页码:305-314
ISSN	0090-6964
摘要	There is increased interest in measuring kinetic rates, lifetimes, and rupture forces of single receptor/ligand bonds. Valuable insights have been obtained from previous experiments attempting such measurements. However, it remains difficult to know with sufficient certainty that single bonds were indeed measured. Using exemplifying data, evidence supporting single-bond observation is examined and caveats in the experimental design and data interpretation are identified. Critical issues preventing definitive proof and disproof of single-bond observation include complex binding schemes, multimeric interactions, clustering, and heterogeneous surfaces. It is concluded that no single criterion is sufficient to ensure that single bonds are actually observed. However, a cumulative body of evidence may provide reasonable confidence. 0 2002 Biomedical Engineering Society.
学科领域	力学
DOI	10.1114/1.1467923
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000175849500004
关键词[WOS]	SELECTIN GLYCOPROTEIN LIGAND-1 ; ATOMIC-FORCE MICROSCOPY ; SHEAR-FLOW ; CELL-ADHESION ; P-SELECTIN ; MOLECULAR ADHESION ; KINETIC RATES ; BINDING ; LIFETIME ; MEMBRANE
WOS研究方向	Engineering
WOS类目	Engineering, Biomedical
引用统计	被引频次：76[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/15995
专题	力学所知识产出(1956-2008)
通讯作者	Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
推荐引用方式 GB/T 7714	Zhu C,Long M,Chesla SE,et al. Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues[J]. Annals of Biomedical Engineering,2002,30,3,:305-314.
APA	朱承,龙勉,Chesla SE,Bongrand P,&Zhu, C .(2002).Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues.Annals of Biomedical Engineering,30(3),305-314.
MLA	朱承,et al."Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues".Annals of Biomedical Engineering 30.3(2002):305-314.

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