Difference in Enzyme Activity and Conformation of Glucose Oxidase Before and After Purification

doi:10.1016/S0927-7765(01)00239-9

IMECH-IR > 力学所知识产出(1956-2008)

	Difference in Enzyme Activity and Conformation of Glucose Oxidase Before and After Purification
	Dai GL(戴国亮); Li JR(李津如); Jiang L(江龙)
发表期刊	Colloids and Surfaces B-Biointerfaces
	2002
卷号	24 期号:3 页码:171-176
ISSN	0927-7765
摘要	EEnzyme activity of commercial glucose oxidase was enhanced after purification through a strong anionic exchange resin. In order to get a better insight into this phenomenon, surface pressure–area ( –A) isotherms and surface pressure–time ( –t) isotherms was used to study the interaction and the absorption at different pH values of the subphases between octadecylamine and glucose oxidase purified by a styrene system quaternary ammonium type strongly basic anionic exchange resin. Circular dichroism (CD), electrophoresis and enzyme activity measurements were conducted to study these phenomena. A preliminary hypothesis has been suggested to explain why the enzyme activity of purified glucose oxidase was higher than that of the commercial one. © 2002 Elsevier Science B.V. All rights reserved.
学科领域	力学
DOI	10.1016/S0927-7765(01)00239-9
收录类别	SCI
语种	英语
WOS记录号	WOS:000174555700001
关键词[WOS]	INTERFACE
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/16857
专题	力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Dai GL,Li JR,Jiang L. Difference in Enzyme Activity and Conformation of Glucose Oxidase Before and After Purification[J]. Colloids and Surfaces B-Biointerfaces,2002,24,3,:171-176.
APA	戴国亮,李津如,&江龙.(2002).Difference in Enzyme Activity and Conformation of Glucose Oxidase Before and After Purification.Colloids and Surfaces B-Biointerfaces,24(3),171-176.
MLA	戴国亮,et al."Difference in Enzyme Activity and Conformation of Glucose Oxidase Before and After Purification".Colloids and Surfaces B-Biointerfaces 24.3(2002):171-176.

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