Application of Optical Protein-chip in Detecting Phage M13KO7

IMECH-IR > 力学所知识产出(1956-2008)

	Application of Optical Protein-chip in Detecting Phage M13KO7
其他题名	光学蛋白芯片技术在噬菌体M13KO7检测中的应用
	Qi C(齐财); Feng J(冯静); Wang ZH(王战会); Meng YH(孟永宏); Yan XY(阎锡蕴); Jin G(靳刚)
发表期刊	CHINESE JOURNAL OF BIOTECHNOLOGY,2006,
	2006
卷号	22 期号:5 页码:856-860
ISSN	1000-3061
摘要	Abstract: Avidin layer was bound on the substrate surface of Silicon wafer modified with aldehyde. The interaction between avidin and biotin was adopted for the immobilization of mouse monoclonal biotin-anti-M13 (antibody GP3)-labeled biotin. The surface was incubated in a solution containing phage M13KO7, which was trapped by the antibody GP3 with the interaction between phage M13KO7 and antibody GP3, resulting in a variation of layer thickness that was detected by imaging ellipsometry. The results showed a saturated layer of antibody GP3 with a thickness about 6.9 nm on the surface of the silicon wafer. The specific interaction between phage M13KO7 and antibody GP3 resulted in a variation of layer thickness. The layer of phage M13KO7 bound with antibody GP3 was 17.5 nm in the concentration of 1.1×1010 pfu/mL. Each variation of the layer thickness corresponded to a concentration of phage M13KO7 in the range of 0.1×1010–2.5×1010 pfu/mL, with the sensitivity of 109 pfu/mL. Compared with other methods, the optical protein-chip, requiring only short measurement time, label free, is a quantitative test, and can be visualized. This study could be significant on the interactions between the antibody and the virus, showing potential in the early diagnosis of virosis.
学科领域	力学
收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/17293
专题	力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Qi C,Feng J,Wang ZH,et al. Application of Optical Protein-chip in Detecting Phage M13KO7[J]. CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,2006,22,5,:856-860.
APA	Qi C,Feng J,Wang ZH,Meng YH,Yan XY,&Jin G.(2006).Application of Optical Protein-chip in Detecting Phage M13KO7.CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,22(5),856-860.
MLA	Qi C,et al."Application of Optical Protein-chip in Detecting Phage M13KO7".CHINESE JOURNAL OF BIOTECHNOLOGY,2006, 22.5(2006):856-860.

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