Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry

doi:10.1016/S0927-7765(02)00133-9

IMECH-IR > 力学所知识产出(1956-2008)

	Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry
	Ying PQ(应佩青); Yu Y(于泳); Jin G(靳刚); Tao ZL(陶祖莱); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
发表期刊	Colloids and Surfaces B-Biointerfaces
	2003
卷号	32 期号:1 页码:1-10
ISSN	0927-7765
摘要	The adsorption and competitive adsorption of collagen and bovine serum albumin (BSA) were directly visualized and quantified using atomic force microscopy (AFM) and imaging ellipsometry. Chemically modified silicon surfaces were used as hydrophilic and hydrophobic substrates. The results showed that collagen and BSA in single component solution adsorbed onto a hydrophobic surface two times more than that onto a hydrophilic surface. The competitive adsorption between collagen and BSA showed that serum albumin preferentially adsorbed onto a hydrophobic surface, while collagen on a hydrophilic surface. In the binary solution of BSA (1 mg/ml BSA) and collagen (0.1 mg/ml), nearly 100% of the protein adsorbed onto the hydrophobic surface was BSA, but on the hydrophilic surface only about 6% was BSA. Surface affinity was the main factor controlling the competitive adsorption.
学科领域	力学
DOI	10.1016/S0927-7765(02)00133-9
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000185498800001
关键词[WOS]	EPITHELIAL-CELLS ; SERUM-ALBUMIN ; SOLUTION AIR ; COLLAGEN ; INTERFACES ; SURFACES ; ADHESION ; CULTURE
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/17322
专题	力学所知识产出(1956-2008)
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
推荐引用方式 GB/T 7714	Ying PQ,Yu Y,Jin G,et al. Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry[J]. Colloids and Surfaces B-Biointerfaces,2003,32,1,:1-10.
APA	应佩青,于泳,靳刚,陶祖莱,&Jin, G .(2003).Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry.Colloids and Surfaces B-Biointerfaces,32(1),1-10.
MLA	应佩青,et al."Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry".Colloids and Surfaces B-Biointerfaces 32.1(2003):1-10.

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