Double spiral microchannel for label-free tumor cell separation and enrichment

doi:10.1039/c2lc40679a

IMECH-IR > 非线性力学国家重点实验室

	Double spiral microchannel for label-free tumor cell separation and enrichment
	Sun JS; Li MM; Liu C(刘超); Zhang Y; Liu DB; Liu WW; Hu GQ(胡国庆); Jiang XY; Sun, JS (reprint author), Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
发表期刊	LAB ON A CHIP
	2012-06
卷号	12 期号:20 页码:3952-3960
ISSN	1473-0197
摘要	This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate the Dean flow inside the curved geometry and to track the particle/cell trajectories, which is validated against the experimental observations and serves as a theoretical foundation for optimizing the operating conditions. Results from separating tumor cells (MCF-7 and Hela) spiked into whole blood indicate that 92.28% of blood cells and 96.77% of tumor cells are collected at the inner and the middle outlet, respectively, with 88.5% tumor recovery rate at a throughput of 3.33 x 10(7) cells min(-1). We expect that this label-free microfluidic platform, driven by purely hydrodynamic forces, would have an impact on fundamental and clinical studies of circulating tumor cells.
学科领域	微机电系统中的流体力学问题
DOI	10.1039/c2lc40679a
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000308894600016
关键词[WOS]	METASTATIC BREAST-CANCER; PERIPHERAL-BLOOD; DIELECTROPHORETIC SEPARATION; MICROFLUIDIC DEVICES; CARCINOMA-CELLS; PARTICLES; CHIP; SIZE; FLOW; TECHNOLOGY
项目资助者	MOST [2011CB933201, 2009CB930001, 2011CB707604]; NSFC [51105086, 21025520, 90813032, 50890182]; CAS [KJCX2-YW-M15]
课题组名称	LNM微纳米流体力学
论文分区	一类
引用统计	被引频次：223[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/47111
专题	非线性力学国家重点实验室
通讯作者	Sun, JS (reprint author), Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Sun JS,Li MM,Liu C,et al. Double spiral microchannel for label-free tumor cell separation and enrichment[J]. LAB ON A CHIP,2012,12,20,:3952-3960.
APA	Sun JS.,Li MM.,Liu C.,Zhang Y.,Liu DB.,...&Sun, JS .(2012).Double spiral microchannel for label-free tumor cell separation and enrichment.LAB ON A CHIP,12(20),3952-3960.
MLA	Sun JS,et al."Double spiral microchannel for label-free tumor cell separation and enrichment".LAB ON A CHIP 12.20(2012):3952-3960.

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