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Title:
Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows
Author: Liu C(刘超); Guo, Jiayi; Tian, Fei; Yang, Na; Yan, Fusheng; Ding, Yanping; Wei, JingYan; Hu GQ(胡国庆); Nie, Guangjun; Sun, Jiashu
Source: ACS NANO
Issued Date: 2017-07-01
Volume: 11, Issue:7, Pages:6968-6976
Abstract: Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size -dependent, and label -free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.
Keyword: exosomes ; microvesicles ; separation ; viscoelasticity ; microfluidics
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Hu, GQ (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. ; Nie, GJ ; Sun, JS (reprint author), Natl Ctr Nanoscience & Technol, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China. ; Hu, GQ ; Nie, GJ ; Sun, JS (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
Correspondent Email: guoqing.hu@imech.ac.cn ; niegj@nanoctr.cn ; sunjs@nanoctr.cn
DOI: 10.1021/acsnano.7b02277
Related URLs: 查看原文
DOC Type: Article
WOS Subject: Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Subject Extended: Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS Keyword Plus: POISEUILLE FLOW ; HIGH-THROUGHPUT ; INERTIAL LIFT ; SEPARATION ; MICROCHANNELS ; PARTICLE ; MICROVESICLES ; CANCER ; FLUIDS ; BLOOD
WOS ID: WOS:000406649700045
ISSN: 1936-0851
Funder: NSFC(11572334 ; CAS Key Research Program of Frontier Sciences(QYZDB-SSW-JSC036) ; CAS Strategic Priority Research Program(XDB22040403) ; Youth Innovation Promotion Association CAS(2016035) ; Beijing Municipal Science & Technology Commission(Z161100000116035) ; 91543125 ; 21622503 ; 21475028 ; U1505228)
Department: LNM微纳米流体力学
Classification: 一类
Ranking: 1
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/70026
Appears in Collections:非线性力学国家重点实验室_期刊论文

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Recommended Citation:
Liu C,Guo, Jiayi,Tian, Fei,et al. Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows[J]. ACS NANO,2017-07-01,11(7):6968-6976.
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