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Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows
Liu C(刘超); Guo, Jiayi; Tian, Fei; Yang, Na; Yan, Fusheng; Ding, Yanping; Wei, JingYan; Hu GQ(胡国庆); Nie, Guangjun; Sun, Jiashu; Hu, GQ (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China.; Nie, GJ; Sun, JS (reprint author), Natl Ctr Nanoscience & Technol, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China.; Hu, GQ; Sun, JS (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
2017-07-01
发表期刊ACS NANO
卷号11期号:7页码:6968-6976
ISSN1936-0851
摘要Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size -dependent, and label -free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.
关键词Exosomes Microvesicles Separation Viscoelasticity Microfluidics
DOI10.1021/acsnano.7b02277
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收录类别SCI ; EI
语种英语
WOS记录号WOS:000406649700045
关键词[WOS]POISEUILLE FLOW ; HIGH-THROUGHPUT ; INERTIAL LIFT ; SEPARATION ; MICROCHANNELS ; PARTICLE ; MICROVESICLES ; CANCER ; FLUIDS ; BLOOD
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
项目资助者NSFC(11572334 ; CAS Key Research Program of Frontier Sciences(QYZDB-SSW-JSC036) ; CAS Strategic Priority Research Program(XDB22040403) ; Youth Innovation Promotion Association CAS(2016035) ; Beijing Municipal Science & Technology Commission(Z161100000116035) ; 91543125 ; 21622503 ; 21475028 ; U1505228)
课题组名称LNM微纳米流体力学
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力学所作者排名1
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被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/70026
专题非线性力学国家重点实验室
通讯作者Hu, GQ (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China.; Nie, GJ; Sun, JS (reprint author), Natl Ctr Nanoscience & Technol, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China.; Hu, GQ; Sun, JS (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
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Liu C,Guo, Jiayi,Tian, Fei,et al. Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows[J]. ACS NANO,2017,11(7):6968-6976.
APA 刘超.,Guo, Jiayi.,Tian, Fei.,Yang, Na.,Yan, Fusheng.,...&Sun, JS .(2017).Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows.ACS NANO,11(7),6968-6976.
MLA 刘超,et al."Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows".ACS NANO 11.7(2017):6968-6976.
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