Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry | |
Liu HY(刘浩雨)1,2; Liu W(刘巍)1; Jin G(靳刚)1,2 | |
发表期刊 | BIOSENSORS-BASEL |
2021-05-01 | |
卷号 | 11期号:5页码:10 |
摘要 | Exosomes are a kind of membrane-bound phospholipid nanovesicle that are secreted extensively in a variety of biological fluids. Accumulating evidence has indicated that exosomes not only communicate with cells, but also perform functional roles in physiology and pathology. In addition, exosomes have also elicited a great deal of excitement due to their potential as disease biomarkers. Therefore, requirements for sensitive methods capable of precisely and specifically determining exosomes were needed. Herein, we not only develop a sensing surface to capture exosomes but also compare two surface proteins on exosomes, which are appropriate for detecting exosome surface markers by total internal reflected imaging ellipsometry (TIRIE). Protein G and antibody were immobilized on a thin layer of golden substrate to form the biosensing surface. The bio-interaction between antibodies and exosomes was recorded by the TIRIE in real time. The distance between exosomes adhered on a surface was 44 nm +/- 0.5 nm. The KD of anti-CD9 and exosome was lower than anti-CD63 and exosome by introducing pseudo-first-order interaction kinetics, which suggested that CD9 is more suitable for exosome surface markers than CD63. The limit of detection (LOD) of TIRIE was 0.4 mu g/mL. In conclusion, we have proposed a surface for the detection of exosomes based on TIRIE, which can make the detection of exosomes convenient and efficient. |
关键词 | TIRIE exosomes surface protein CD9 CD63 interaction kinetics |
DOI | 10.3390/bios11050164 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000653707800001 |
关键词[WOS] | EXTRACELLULAR VESICLES ; SPR DETECTION ; PROTEINS ; IMMUNOSENSOR |
WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
WOS类目 | Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation |
论文分区 | 二类 |
力学所作者排名 | 1 |
RpAuthor | Jin, Gang |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/86700 |
专题 | 微重力重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China; 2.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Liu HY,Liu W,Jin G. Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry[J]. BIOSENSORS-BASEL,2021,11,5,:10. |
APA | Liu HY,Liu W,&Jin G.(2021).Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry.BIOSENSORS-BASEL,11(5),10. |
MLA | Liu HY,et al."Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry".BIOSENSORS-BASEL 11.5(2021):10. |
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