| Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces | |
Almeida, Luis C.1; Frade, Tania1; Correia, Rui D.1; Niu Y(牛宇)2; Jin G(靳刚)2 ; Correia, Jorge P.1; Viana, Ana S.1
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| Corresponding Author | Viana, Ana S.(anaviana@fc.ul.pt) |
| Source Publication | SCIENTIFIC REPORTS
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| 2021-01-26 | |
| Volume | 11Issue:1Pages:12 |
| ISSN | 2045-2322 |
| Abstract | We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection. |
| DOI | 10.1038/s41598-021-81816-1 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000667507900024 |
| WOS Keyword | SURFACE-CHEMISTRY ; CARBON-DISULFIDE ; DOPAMINE ; ADSORPTION ; LACCASE ; IMMOBILIZATION ; NANOPARTICLES ; OXIDATION ; OXIDE ; TOOL |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Project | Fundacao para a Ciencia e Tecnologia (FCT)[UIDB/00100/2020] ; Fundacao para a Ciencia e Tecnologia (FCT)[SFRH/BD/129566/2017] ; Fundacao para a Ciencia e Tecnologia (FCT)[PTDC/CTM-NAN/0994/2014] |
| Funding Organization | Fundacao para a Ciencia e Tecnologia (FCT) |
| Classification | 二类/Q1 |
| Ranking | 3+ |
| Contributor | Viana, Ana S. |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/87032 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Univ Lisbon, Fac Ciencias, Ctr Quim Estrutural, P-1749016 Lisbon, Portugal; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, NML, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Almeida, Luis C.,Frade, Tania,Correia, Rui D.,et al. Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces[J]. SCIENTIFIC REPORTS,2021,11,1,:12. |
| APA | Almeida, Luis C..,Frade, Tania.,Correia, Rui D..,牛宇.,靳刚.,...&Viana, Ana S..(2021).Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces.SCIENTIFIC REPORTS,11(1),12. |
| MLA | Almeida, Luis C.,et al."Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces".SCIENTIFIC REPORTS 11.1(2021):12. |
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| Jp2021F263.pdf（2611KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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