Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors

doi:10.1007/s11433-021-1752-0

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	Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors
	Li L(李龙)1,2 ; Hu, Jinglei 3,4,5; Wu, Huaping 6; Song F(宋凡)1,2,7
通讯作者	Hu, Jinglei(hujinglei@nju.edu.cn) ; Wu, Huaping(wuhuaping@gmail.com) ; Song, Fan(songf@lnm.imech.ac.cn)
发表期刊	SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
	2021-10-01
卷号	64 期号:10 页码:10
ISSN	1674-7348
摘要	Cell adhesion on a supported lipid bilayer (SLB) functionalized with ligand proteins is a widely-used cell-mimetic model for the study of the trans receptor-ligand interaction that mediates the adhesion, and has been shown to play an important role in unravelling the molecular players in cell adhesion. Experimental studies of such cell-SLB adhesion systems often assume that there is no cis interactions between the ligands. An important question remains whether the cis-interaction affects the trans receptor-ligand interaction. Using a statistical-mechanical model and Monte Carlo simulations with biologically relevant parameters, we find that the attractive cis-interaction of strength 1 k(B)T between adjacent ligands on the SLB can lead to an amplification of both the affinity and cooperativity of the receptor-ligand binding, thereby facilitating the phase separation within the adhering cell membrane. In contrast, the adhesion system is less sensitive to the repulsive cis-interaction between adjacent ligands on the SLB. Our results suggest that the ligand-ligand cis-interaction should be carefully considered in the cell-SLB adhesion experiments.
关键词	cell adhesion ligand-ligand cis-interaction receptor-ligand interaction phase behavior
DOI	10.1007/s11433-021-1752-0
收录类别	SCI ; EI ; CSCD
语种	英语
WOS记录号	WOS:000693207100001
关键词[WOS]	MEMBRANE-ANCHORED RECEPTORS ; RAFTS ; DIFFUSION ; CONSTANTS ; CLUSTERS ; KINETICS ; MODELS
WOS研究方向	Physics
WOS类目	Physics, Multidisciplinary
资助项目	National Natural Science Foundation of China[11902327] ; National Natural Science Foundation of China[21973040] ; National Natural Science Foundation of China[11972041] ; Youth Innovation Promotion Association of Chinese Academy of Sciences, Strategic Priority Research Program of the Chinese Academy of Sciences[XDB22040102] ; Fundamental Research Funds for the Central Universities[14380228] ; Shenzhen Science and Technology Innovation Committee[JCYJ20200109150656717] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170818110613113]
项目资助者	National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Shenzhen Science and Technology Innovation Committee
论文分区	二类/Q1
力学所作者排名	1
RpAuthor	Hu, Jinglei ; Wu, Huaping ; Song, Fan
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/87409
专题	非线性力学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Nanjing Univ, Kuang Yaming Honors Sch, Nanjing 210023, Peoples R China; 4.Nanjing Univ, Inst Brain Sci, Nanjing 210023, Peoples R China; 5.Nanjing Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China; 6.Zhejiang Univ Technol, Coll Mech Engn, Hangzhou 310023, Peoples R China; 7.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li L,Hu, Jinglei,Wu, Huaping,et al. Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2021,64,10,:10.
APA	李龙,Hu, Jinglei,Wu, Huaping,&宋凡.(2021).Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,64(10),10.
MLA	李龙,et al."Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 64.10(2021):10.

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