IMECH-IR  > 微重力重点实验室
Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip
Huang,Chenghong; Tang,Hong; Chen,Hongjie; Huang,Xiaorong; Fang,Ying; Wang,KangRui; Men,Rongrong; Gao,Jie; Wang,Yang; Chen YY(陈艳艳); Jin G(靳刚)
Source PublicationINTEGRATED FERROELECTRICS
2022
Pages148-155
Conference Name20th China International Nanoscience and Technology Symposium / Nano-Products Exposition
Conference DateOCT 22-25, 2021
Conference PlaceXian, PEOPLES R CHINA
AbstractFor immunoassay, immobilization of antibody is the most important technique in bioengineering for maintaining its intrinsic activity of antigen binding. The sugar chain in the Fc region carried by antibody can be oxidized into aldehyde group by sodium m-periodate, which can couple with the amino-group on chemistry modified substrate surface through covalent linking. Thereby, in situ site-directed immobilization of the oxidized antibody for only one-step reaction can be performed. Here we propose a facile method that oxidized anti-human alpha-fetoprotein antibody (anti-AFP) was in situ site-directed immobilized on APTES treated silicon substrate utilizing microfluidic channel system for AFP immunoassay. The shape of the immobilized anti-AFP was observed by AFM and imaged by imaging ellipsometry of self-developed protein system. Results shown that the amount of antibody immobilization amount and capacity of antigen binding of random immobilization and site-directed immobilization were separately enhanced 16.0% and 7.0%. the suggested strategy has the advantages of simple, environment-friend and convenient-operation.
Keywordprotein chip alpha-fetoprotein site-directed immobilization of antibody
WOS IDWOS:000805668600015
Funding OrganizationNSF of Chongqing [cstc2019jscx-msxm0366]; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism [GYFJWSW-201905]; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJZD-K201806401, KJZD-K201906401]; graduate Innovation Fund of CQUST [YKJCX2020509, YKJCX2120502, YKJCX2120541, 202111551006]
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Indexed ByCPCI-S
Language英语
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Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/90524
Collection微重力重点实验室
Affiliation1.CQUST, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China
2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China
3.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Huang,Chenghong,Tang,Hong,Chen,Hongjie,et al. Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip[C]INTEGRATED FERROELECTRICS,2022:148-155.
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