Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells

doi:10.3390/bioengineering10030384

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	Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
	Cao, Jialing 1; Li HY(李航宇); Tang, Hongyan ; Gu, Xuenan ; Wang, Yan 4; Guan DS(关东石); Du, Jing ; Fan, Yubo
发表期刊	BIOENGINEERING BASEL
	2023-03
卷号	10 期号:3 页码:384
摘要	The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell ECM interactions. The mechanical microenvironment of the uterus was simulated by fabricating polyacrylamide (PA) hydrogels with different levels of stiffness. The human choriocarcinoma (JAR) cell lineage was used as the trophoblast model. We found that the spreading area of JAR cells, the formation of focal adhesions, and the polymerization of the F actin cytoskeleton were all facilitated with increased ECM stiffness. Significantly, JAR cells also exhibited durotactic behavior on ECM with a gradient stiffness. Meanwhile, stiffness of the ECM affects the invasion of multicellular JAR spheroids. These results demonstrated that human trophoblast cells are mechanically sensitive, while the mechanical properties of the uterine microenvironment could play an important role in the implantation process.
关键词	embryo implantation human choriocarcinoma cell extracellular matrix stiffness durotaxis
DOI	10.3390/bioengineering10030384
收录类别	SCI
语种	英语
WOS记录号	WOS:000957330500001
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering, Biomedical
WOS类目	Biotechnology & Applied Microbiology ; Engineering
项目资助者	National Natural Science Foundation of China [12222201, 82273500, U20A20390, 11827803, 11972351] ; Fundamental Research Funds for the Central Universities [ZG140S1971] ; National Key R&D Program of China [2021YFA0719302] ; Peking University Health Science Center X Cross Seed Fund [BMU2020MX014] ; Incubation fund for reserve candidates of Peking University Third Hospital [BYSYFY2021007]
论文分区	二类
力学所作者排名	1
RpAuthor	Du, J ; Fan, YB (corresponding author), Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China. ; Guan, DS (corresponding author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. ; Guan, DS (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. ; Wang, Y (corresponding author), Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China.
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/91791
专题	非线性力学国家重点实验室
作者单位	1.Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China 2.Beihang Univ, Sino French Engineer Sch, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 5.Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Cao, Jialing,Li HY,Tang, Hongyan,et al. Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells[J]. BIOENGINEERING BASEL,2023,10,3,:384.
APA	Cao, Jialing.,李航宇.,Tang, Hongyan.,Gu, Xuenan.,Wang, Yan.,...&Fan, Yubo.(2023).Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells.BIOENGINEERING BASEL,10(3),384.
MLA	Cao, Jialing,et al."Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells".BIOENGINEERING BASEL 10.3(2023):384.

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