Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry

doi:10.1016/j.tsf.2022.139578

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	Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry
	Wang CY(王晨昱); Liu W(刘巍); Niu Y(牛宇); Xu P(徐鹏); Jin G(靳刚)
发表期刊	THIN SOLID FILMS
	2022-12-01
卷号	763 页码:139578
ISSN	0040-6090
摘要	The biosensor based on imaging ellipsometry (BIE) is a practical technique to measure protein interactions such as the immobilization of biomolecules and the recognition between ligand and analyte. In this paper, to avoid the working condition optimization before each measurement, a fixed linear working condition for the silicon wafer chip is established at the angle of incidence of 75 degrees with the fixed azimuths of the polarizer and the analyzer P = 90 degrees and A = 45 degrees. Further, a quantitative model is constructed to establish the standard curve between the ellipsometric signal and the protein concentration. As a result, this model is used to deduce the dissociation equilibrium constants of three relevant blood proteins as well as their antibodies. This work bridges the gap between the biochemical demand and BIE for sensing applications.
关键词	Imaging ellipsometry Biosensor Quantitative detection
DOI	10.1016/j.tsf.2022.139578
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000950649200001
WOS研究方向	Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
WOS类目	Materials Science ; Physics
项目资助者	National Natural Science Foundation of China [81872584]
论文分区	Q3
力学所作者排名	1
RpAuthor	Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. ; Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/91817
专题	微重力重点实验室
作者单位	1.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 4.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Foundamenal Phys & Math Sci, Hangzhou 310024, Peoples R China
推荐引用方式 GB/T 7714	Wang CY,Liu W,Niu Y,et al. Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry[J]. THIN SOLID FILMS,2022,763:139578.
APA	王晨昱,刘巍,牛宇,徐鹏,&靳刚.(2022).Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry.THIN SOLID FILMS,763,139578.
MLA	王晨昱,et al."Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry".THIN SOLID FILMS 763(2022):139578.

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