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Effects of forced solution flow on lysozyme crystal growth
Yu Y(于泳); Liu YH(刘玉红); Wang X(王翾); Oberthür D; Dierks K; Betzel C; Yong Yu(于泳)
Source PublicationCrystal Research and Technology
2010
Volume45Issue:4Pages:380-386
ISSN0232-1300
AbstractIn order to study quantitatively the effects of forced solution on crystal growth, we designed a new set of experimental equipment, in particular, a microchannel mixer was used as crystallization container so that the consumption of protein samples was much reduced and thus an exact syringe pump could be used for precise control of the flow rates. Since the mixer’s section was designed to be rectangular, the solution velocity in its center was steady and constant, and thus repeatable experiments were facilitated. Experimental results showed that the effects of forced solution on protein crystal growth were different under different levels of supersaturation, and new results were obtained for cases of high supersaturation. When the supersaturation is σ = 2.3, with increasing flow rates the growth rates of the lysozyme crystal’s (110) face hardly change when the flow rates are lower than 1300 μm/s, and decrease quickly afterwards. When the flow rate reaches 2000 μm/s, the crystal nearly ceases to grow. When the supersaturation is σ = 2.7, with increasing flow rates the (110) face growth rates increase at the beginning then reach the maximum values at 1700 μm/s – 1900 μm/s and decrease afterwards, approaching zero or so when the flow rate reaches 12000 μm/s. The higher the supersaturation, the larger the flow rate at which the crystal ceases to grow. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
KeywordProtein Crystallization Fluid Flow Impurities Mass Transfer Lysozyme.
Subject Area交叉与边缘领域的力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000276599200007
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/43244
Collection国家微重力实验室
Corresponding AuthorYong Yu(于泳)
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GB/T 7714
Yu Y,Liu YH,Wang X,et al. Effects of forced solution flow on lysozyme crystal growth[J]. Crystal Research and Technology,2010,45(4):380-386.
APA Yu Y.,Liu YH.,Wang X.,Oberthür D.,Dierks K.,...&Yong Yu.(2010).Effects of forced solution flow on lysozyme crystal growth.Crystal Research and Technology,45(4),380-386.
MLA Yu Y,et al."Effects of forced solution flow on lysozyme crystal growth".Crystal Research and Technology 45.4(2010):380-386.
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