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Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow
Baik AD; Lu XL; Qiu J; Huo B(霍波); Hillman EMC; Dong C; Guo XE; Huo B
Source PublicationBiophysical Journal
AbstractOsteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption. Whole-cell deformation and regional deformation of the cytoskeleton may be able to directly regulate this process. Attempts to image cellular deformation by conventional microscopy techniques have been hindered by low temporal or spatial resolution. In this study, we developed a quasi-three-dimensional microscopy technique that enabled us to simultaneously visualize an osteocyte's traditional bottom-view profile and a side-view profile at high temporal resolution. Quantitative analysis of the plasma membrane and either the intracellular actin or microtubule (MT) cytoskeletal networks provided characterization of their deformations over time. Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components. Regional heterogeneity in the strain field of normal strains was observed in the actin networks, especially in the leading edge to flow, but not in the MT networks. In contrast, side-view shear strains exhibited similar subcellular distribution patterns in both networks. Disruption of MT networks caused actin normal strains to decrease, whereas actin disruption had little effect on the MT network strains, highlighting the networks' mechanical interactions in osteocytes.
KeywordLiving Endothelial-cells Viscoelastic Properties Articular-cartilage Shear-stress Bone-cells In-vitro Adhesion Deformation Prestress Strain
Indexed BySCI
WOS IDWOS:000283912600015
WOS Research AreaBiophysics
WOS SubjectBiophysics
Funding OrganizationNational Institutes of Health [AR058453, AR052417]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorHuo B
Recommended Citation
GB/T 7714
Baik AD,Lu XL,Qiu J,et al. Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow[J]. Biophysical Journal,2010,99(9):2812-2820.
APA Baik AD.,Lu XL.,Qiu J.,霍波.,Hillman EMC.,...&Huo B.(2010).Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow.Biophysical Journal,99(9),2812-2820.
MLA Baik AD,et al."Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow".Biophysical Journal 99.9(2010):2812-2820.
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