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The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates
Li N(李宁); Zhang XM; Song Q; Su RG(苏瑞巩); Zhang Q; Kong T; Liu LW; Jin G(靳刚); Tang ML; Cheng GS; Tang, ML (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, 398 Ruoshui Rd, Suzhou Ind Pk 215123, Peoples R China
Source PublicationBiomaterials
2011
Volume32Issue:35Pages:9374-9382
ISSN0142-9612
AbstractGraphene has been demonstrated in many biomedical applications and its potentials for neural interfacing. Emerging concerns on graphene, as a biomedical material, are its biocompatibility and how biologically targeted tissue/cells respond to it. Relatively few studies attempted to address the interactions of graphene or its derivatives with the tissues/cells, while very few reports on neural system. In this study, we tried to explore how neurites, one of the key structures for neural functions, are affected by graphene during the development until maturation in a mouse hippocampal culture model. The results reveal that graphene substrates exhibited excellent biocompatibility, as cell viability and morphology were not affected. Meanwhile, neurite numbers and average neurite length on graphene were significantly enhanced during 2-7 days after cell seeding compared with tissue culture polystyrene (TCPS) substrates. Especially on Day 2 of the neural development period, graphene substrates efficiently promoted neurite sprouting and outgrowth to the maximal extent. Additionally, expression of growth-associate protein-43 (GAP-43) was examined in both graphene and TCPS groups. Western blot analysis showed that GAP-43 expression was greatly enhanced in graphene group compared to TCPS group, which might result in the boost of neurite sprouting and outgrowth. This study suggests the potential of graphene as a material for neural interfacing and provides insight into the future biomedical applications of graphene. (C) 2011 Elsevier Ltd. All rights reserved.
KeywordNeuron-favorable Cvd Graphene Gap-43 Neurite Sprouting & Outgrowth Hippocampus Few-layer Graphene Carbon Nanotubes Neuronal Growth Large-area Oxide Interfaces Films Cytotoxicity Delivery Systems
Subject AreaEngineering ; Materials Science
DOI10.1016/j.biomaterials.2011.08.065
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000296684200023
WOS KeywordFEW-LAYER GRAPHENE ; CARBON NANOTUBES ; NEURONAL GROWTH ; LARGE-AREA ; OXIDE ; INTERFACES ; FILMS ; CYTOTOXICITY ; DELIVERY ; SYSTEMS
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
Funding OrganizationThis work was partially funded by National Basic Research Program of China under award numbers of 2011CB965004, Fund for Presidential Award Winners of Chinese Academy of Sciences (Y1BFS11001) and "100-Talents Program" of Chinese Academy of Sciences. This work was made possible by extraordinarily professional services by Nano-Fabrication & Nano-Characterization Platforms with Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences. We thank Mr. Weiwei Li for the help of graphene synthesis.
DepartmentNML纳米生物光学
Classification一类
Citation statistics
Cited Times:247[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45065
Collection国家微重力实验室
Corresponding AuthorTang, ML (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, 398 Ruoshui Rd, Suzhou Ind Pk 215123, Peoples R China
Recommended Citation
GB/T 7714
Li N,Zhang XM,Song Q,et al. The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates[J]. Biomaterials,2011,32(35):9374-9382.
APA 李宁.,Zhang XM.,Song Q.,苏瑞巩.,Zhang Q.,...&Tang, ML .(2011).The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates.Biomaterials,32(35),9374-9382.
MLA 李宁,et al."The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates".Biomaterials 32.35(2011):9374-9382.
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