Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow

doi:10.1007/s12195-011-0167-x

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	Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow
	Fu ZL(傅长亮); Tong CF(佟春芳); Dong C; Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
发表期刊	Cellular and Molecular Bioengineering
	2011
卷号	4 期号:3 页码:427-441
ISSN	1865-5025
摘要	Shear-induced cell aggregation and disaggregation, governed by specific receptor-ligand binding, play important roles in many biological and biophysical processes. While a lot of studies have focused on elucidating the shear rate and shear stress dependence of cell aggregation, the majority of existing models based on population balance equation (PBE) has rarely dealt with cell aggregation dynamics upon intrinsic molecular kinetics. Here, a kinetic model was developed for further understanding cell aggregation and disaggregation in a linear shear flow. The novelty of the model is that a set of simple equations was constructed by coupling two-body collision theory with receptor-ligand binding kinetics. Two cases of study were employed to validate the model: one is for the homotypic aggregation dynamics of latex beads cross-linked by protein G-IgG binding, and the other is for the heterotypic aggregation dynamics of neutrophils-tumor cells governed by beta 2-integrin-ligand interactions. It was found that the model fits the data well and the obtained kinetic parameters are consistent with the previous predictions and experimental measurements. Moreover, the decay factor defined biophysically to account for the chemokine- and shear-induced regulation of receptor and/or ligand expression and conformation was compared at molecular and cellular levels. Our results provided a universal framework to quantify the molecular kinetics of receptor-ligand binding in shear-induced cell aggregation dynamics.
关键词	Two-dimensional Kinetics Cone-plate Viscometer Homotypic Aggregation Heterotypic Aggregation Bell Model Protein G-igg Bond Beta(2)-integrin And Icam-1 Bond Human Blood-platelets Intercellular-adhesion Molecule-1 Hydrodynamic Shear Disaggregation Kinetics Neutrophil Aggregation Sequential Binding Mediated Adhesion Stable Adhesion Melanoma-cells Latex Spheres
学科领域	Cell Biology ; Biophysics
DOI	10.1007/s12195-011-0167-x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000297866300011
关键词[WOS]	HUMAN BLOOD-PLATELETS ; INTERCELLULAR-ADHESION MOLECULE-1 ; HYDRODYNAMIC SHEAR ; DISAGGREGATION KINETICS ; NEUTROPHIL AGGREGATION ; SEQUENTIAL BINDING ; MEDIATED ADHESION ; STABLE ADHESION ; MELANOMA-CELLS ; LATEX SPHERES
WOS研究方向	Cell Biology ; Biophysics
WOS类目	Cell & Tissue Engineering ; Biophysics ; Cell Biology
项目资助者	This work was supported by National Natural Science Foundation of China grants 30730032, 11072251, 10902117, and 10702075, Chinese Academy of Sciences grants KJCX2-YW-L08 and Y2010030, and National Key Basic Research Foundation of China grant 2011CB710904.
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	Q4
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45075
专题	微重力重点实验室
通讯作者	Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Fu ZL,Tong CF,Dong C,et al. Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow[J]. Cellular and Molecular Bioengineering,2011,4,3,:427-441.
APA	傅长亮,佟春芳,Dong C,龙勉,&Long, M .(2011).Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow.Cellular and Molecular Bioengineering,4(3),427-441.
MLA	傅长亮,et al."Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow".Cellular and Molecular Bioengineering 4.3(2011):427-441.

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