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Title: Probe stiffness regulates receptor-ligand bond lifetime under force
Author: Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉)
Source: Science China-Physics Mechanics & Astronomy
Issued Date: 2011
Volume: 54, Issue:5, Pages:923-929
Abstract: Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
Keyword: Probe Stiffness ; Bond Lifetime ; Optical Trap ; Selectin Glycoprotein Ligand-1 ; P-Selectin ; Cell-Adhesion ; Binding-Kinetics ; Leukocytes ; Molecule ; Psgl-1 ; Flow
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Correspondent Email: mlong@imech.ac.cn
DOI: 10.1007/s11433-011-4302-4
Related URLs: 查看原文
DOC Type: Article; Proceedings Paper
WOS Subject: Physics, Multidisciplinary
WOS Subject Extended: Physics
WOS Keyword Plus: SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; BINDING-KINETICS ; LEUKOCYTES ; MOLECULE ; PSGL-1 ; FLOW
WOS ID: WOS:000290768300022
ISSN: 1674-7348
Rank: [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China
Subject: Physics
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类/Q3
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/45085
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
章燕;吕守芹;龙勉.Probe stiffness regulates receptor-ligand bond lifetime under force,SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2011,54(5):923-929
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