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Probe stiffness regulates receptor-ligand bond lifetime under force
Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Source PublicationScience China-Physics Mechanics & Astronomy
2011
Volume54Issue:5Pages:923-929
ISSN1674-7348
AbstractReceptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
KeywordProbe Stiffness Bond Lifetime Optical Trap Selectin Glycoprotein Ligand-1 P-selectin Cell-adhesion Binding-kinetics Leukocytes Molecule Psgl-1 Flow
Subject AreaPhysics
DOI10.1007/s11433-011-4302-4
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000290768300022
WOS KeywordSELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; BINDING-KINETICS ; LEUKOCYTES ; MOLECULE ; PSGL-1 ; FLOW
WOS Research AreaPhysics
WOS SubjectPhysics, Multidisciplinary
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类/Q3
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45085
Collection国家微重力实验室
Corresponding AuthorLong, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[J]. Science China-Physics Mechanics & Astronomy,2011,54(5):923-929.
APA 章燕,吕守芹,龙勉,&Long, MA .(2011).Probe stiffness regulates receptor-ligand bond lifetime under force.Science China-Physics Mechanics & Astronomy,54(5),923-929.
MLA 章燕,et al."Probe stiffness regulates receptor-ligand bond lifetime under force".Science China-Physics Mechanics & Astronomy 54.5(2011):923-929.
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