| Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry |
| Niu Y(牛宇) ; Zhuang J; Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
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| 发表期刊 | Thin Solid Films
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| 2011
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| 卷号 | 519期号:9页码:2768-2771 |
| ISSN | 0040-6090
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| 摘要 | In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved. |
| 关键词 | Ricin Detection
Antigen-antibody Interaction
Biosensor
Imaging Ellipsometry
Neutralizing Monoclonal-antibody
Optical Protein-chip
Silicon Surface
Immobilization
Visualization
System
Cells
Assay
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| 学科领域 | Materials Science
; Physics
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| DOI | 10.1016/j.tsf.2010.12.054
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| URL | 查看原文
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| 收录类别 | SCI
; EI
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| 语种 | 英语
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| WOS记录号 | WOS:000289174200043
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| 关键词[WOS] | NEUTRALIZING MONOCLONAL-ANTIBODY
; OPTICAL PROTEIN-CHIP
; SILICON SURFACE
; IMMOBILIZATION
; VISUALIZATION
; SYSTEM
; CELLS
; ASSAY
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| WOS研究方向 | Materials Science
; Physics
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| WOS类目 | Materials Science, Multidisciplinary
; Materials Science, Coatings & Films
; Physics, Applied
; Physics, Condensed Matter
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| 课题组名称 | NML纳米生物光学
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| 论文分区 | 二类/Q2
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/45102
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| 专题 | 微重力重点实验室
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| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
推荐引用方式
GB/T 7714 |
Niu Y,Zhuang J,Liu L,et al. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[J]. Thin Solid Films,2011,519,9,:2768-2771.
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| APA |
牛宇,Zhuang J,刘丽,Yan XY,靳刚,&Jin, G .(2011).Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry.Thin Solid Films,519(9),2768-2771.
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| MLA |
牛宇,et al."Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry".Thin Solid Films 519.9(2011):2768-2771.
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