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A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection
Liu JS; Wang JY; Chen ZG; Yu Y(于泳); Yang XJ; Zhang XB; Xu Z; Liu, JS (reprint author), Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China
Source PublicationLab on A Chip
2011
Volume11Issue:5Pages:969-973
ISSN1473-0197
AbstractA three-layer poly (methyl methacrylate) (PMMA) electrophoresis microchip integrated with Pt microelectrodes for contactless conductivity detection is presented. A 50 mu m-thick PMMA film is used as the insulating layer and placed between the channel plate (containing the microchannel) and the electrode plate (containing the microelectrode). The three-layer structure facilitates the achievement of a thin insulating layer, obviates the difficulty of integrating microelectrodes on a thin film, and does not compromise the integration of microchips. To overcome the thermal and chemical incompatibilities of polymers and photolithographic techniques, a modified lift-off process was developed to integrate Pt microelectrodes onto the PMMA substrate. A novel two-step bonding method was created to assemble the complete PMMA microchip. A low limit of detection of 1.25 mu g ml(-1) for Na(+) and high separation efficiency of 77 000 and 48 000 plates/m for Na(+) and K(+) were obtained when operating the detector at a low excitation frequency of 60 kHz.
KeywordCapillary-electrophoresis Fabrication System Polymerization Electrodes Devices Chip
Subject AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
DOI10.1039/c0lc00341g
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000287409600028
WOS KeywordCAPILLARY-ELECTROPHORESIS ; FABRICATION ; SYSTEM ; POLYMERIZATION ; ELECTRODES ; DEVICES ; CHIP
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
Funding OrganizationThis work was supported by the National Natural Science Foundation of China (50605006, 51075056, 50705012, 20890024, 20727006), and the National Basic Research Program of China (2007CB714502). We thank Yanan Du, Jason W. Nichol and Seila Selimovic for scientific discussions and revisions. J. Liu, Y. Yu, Z. Chen, Z. Xu and C. Liu designed the research strategy; J. Liu, J. Wang, X. Yang and X. Zhang performed the experiments; Y. Yu designed the microchip; J. Liu, J. Wang and X. Yang wrote the paper.
DepartmentNML复杂流体
Classification一类
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45108
Collection国家微重力实验室
Corresponding AuthorLiu, JS (reprint author), Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Liu JS,Wang JY,Chen ZG,et al. A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection[J]. Lab on A Chip,2011,11(5):969-973
APA Liu JS.,Wang JY.,Chen ZG.,Yu Y.,Yang XJ.,...&Liu, JS .(2011).A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection.Lab on A Chip,11(5),969-973.
MLA Liu JS,et al."A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection".Lab on A Chip 11.5(2011):969-973.
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