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Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)
Liu L(刘丽); Chen YY(陈艳艳); Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Source PublicationTHIN SOLID FILMS
2011
Pages2758-2762
Conference Name5th International Conference on Spectroscopic Ellipsometry
Conference DateMAY 23-29, 2010
Conference PlaceAlbany, NY
AbstractThe biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is realized as an automatic analysis method for protein interaction processes in real-time, with high throughput and label-free. An evanescent wave is used as the optical probe to monitor bio-molecular interactions on a chip surface with a high sensitivity due to its phase sensitive property. In this paper, the technique is optimized with a polarization setting, a spectroscopic light source and a low noise CCD detector to improve the performance of the biosensor in sensitivity and detection limit, as evidenced by a quantitative detection of Hepatitis B virus surface antigen (HbsAg) with concentrations of 8, 16, 32, 64, 125 and 250 ng/ml. The sensitivity is increased by one order of magnitude and the detection limit has been extended more than 50 times for HbsAg detection. (C) 2011 Elsevier B.V. All rights reserved.
KeywordSensitivity Improvement Imaging Ellipsometry Total Internal Reflection Biosensor Hbsag Detection
DepartmentNML纳米生物光学
Funding OrganizationUniv Albany, Coll Nanoscale & Engn
URL查看原文
Indexed ByCPCI
Language英语
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45319
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Liu L,Chen YY,Meng YH,et al. Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)[C]THIN SOLID FILMS,2011:2758-2762.
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