Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)

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	Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)
	Liu L(刘丽); Chen YY(陈艳艳); Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
会议录名称	THIN SOLID FILMS
	2011
页码	2758-2762
会议名称	5th International Conference on Spectroscopic Ellipsometry
会议日期	MAY 23-29, 2010
会议地点	Albany, NY
摘要	The biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is realized as an automatic analysis method for protein interaction processes in real-time, with high throughput and label-free. An evanescent wave is used as the optical probe to monitor bio-molecular interactions on a chip surface with a high sensitivity due to its phase sensitive property. In this paper, the technique is optimized with a polarization setting, a spectroscopic light source and a low noise CCD detector to improve the performance of the biosensor in sensitivity and detection limit, as evidenced by a quantitative detection of Hepatitis B virus surface antigen (HbsAg) with concentrations of 8, 16, 32, 64, 125 and 250 ng/ml. The sensitivity is increased by one order of magnitude and the detection limit has been extended more than 50 times for HbsAg detection. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Sensitivity Improvement Imaging Ellipsometry Total Internal Reflection Biosensor Hbsag Detection
课题组名称	NML纳米生物光学
资助信息	Univ Albany, Coll Nanoscale & Engn
URL	查看原文
收录类别	CPCI
语种	英语
文献类型	会议论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45319
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu L,Chen YY,Meng YH,et al. Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)[C]THIN SOLID FILMS,2011:2758-2762.

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