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Design and application of a microfluidic device for protein crystallization using an evaporation-based crystallization technique
Yu, Yong1; Wang, Xuan1; Oberthuer, Dominik2; Meyer, Arne3; Perbandt, Markus2; Duan, Li1; Kang, Qi1; Yu Y(于泳)
Source PublicationJOURNAL OF APPLIED CRYSTALLOGRAPHY
2012-02-01
Volume45Issue:2Pages:53-60
ISSN0021-8898
AbstractA new crystallization system is described, which makes it possible to use an evaporation-based microfluidic crystallization technique for protein crystallization. The gas and water permeability of the used polydimethylsiloxane (PDMS) material enables evaporation of the protein solution in the microfluidic device. The rates of evaporation are controlled by the relative humidity conditions, which are adjusted in a precise and stable way by using saturated solutions of different reagents. The protein crystals could nucleate and grow under different relative humidity conditions. Using this method, crystal growth could be improved so that approximately 1 mm-sized lysozyme crystals were obtained more successfully than using standard methods. The largest lysozyme crystal obtained reached 1.57 mm in size. The disadvantage of the good gas permeability in PDMS microfluidic devices becomes an advantage for protein crystallization. The radius distributions of aggregrates in the solutions inside the described microfluidic devices were derived from in situ dynamic light scattering measurements. The experiments showed that the environment inside of the microfluidic device is more stable than that of conventional crystallization techniques. However, the morphological results showed that the protein crystals grown in the microfluidic device could lose their morphological stability. Air bubbles in microfluidic devices play an important role in the evaporation progress. A model was constructed to analyze the relationship of the rates of evaporation and the growth of air bubbles to the relative humidity.; A new crystallization system is described, which makes it possible to use an evaporation-based microfluidic crystallization technique for protein crystallization. The gas and water permeability of the used polydimethylsiloxane (PDMS) material enables evaporation of the protein solution in the microfluidic device. The rates of evaporation are controlled by the relative humidity conditions, which are adjusted in a precise and stable way by using saturated solutions of different reagents. The protein crystals could nucleate and grow under different relative humidity conditions. Using this method, crystal growth could be improved so that approximately 1 mm-sized lysozyme crystals were obtained more successfully than using standard methods. The largest lysozyme crystal obtained reached 1.57 mm in size. The disadvantage of the good gas permeability in PDMS microfluidic devices becomes an advantage for protein crystallization. The radius distributions of aggregrates in the solutions inside the described microfluidic devices were derived from in situ dynamic light scattering measurements. The experiments showed that the environment inside of the microfluidic device is more stable than that of conventional crystallization techniques. However, the morphological results showed that the protein crystals grown in the microfluidic device could lose their morphological stability. Air bubbles in microfluidic devices play an important role in the evaporation progress. A model was constructed to analyze the relationship of the rates of evaporation and the growth of air bubbles to the relative humidity.
KeywordMicrofluidics Protein Crystallization
Subject Area交叉与边缘领域的力学
DOI10.1107/S0021889811048047
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000299206400007
WOS KeywordVAPOR-DIFFUSION ; CRYSTAL-GROWTH ; OPTIMIZATION ; LYSOZYME ; DROPLETS ; NUCLEATION ; FLOW
WOS Research AreaCrystallography
WOS SubjectCrystallography
Funding OrganizationNational Natural Science Foundation of China(10972224 ; Chinese Academy of Sciences(KJCX2-YW-L08) ; 11032011)
DepartmentNML复杂流体
Classification一类
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45653
Collection国家微重力实验室
Corresponding AuthorYu Y(于泳)
Affiliation1.Chinese Acad Sci, Key Lab Micrograv, Natl Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China
2.Univ Hamburg, Inst Biochem & Mol Biol, DESY, D-22603 Hamburg, Germany
3.Univ Lubeck, Lab Struct Biol Infect & Inflammat, DESY, D-22607 Hamburg, Germany
Recommended Citation
GB/T 7714
Yu, Yong,Wang, Xuan,Oberthuer, Dominik,et al. Design and application of a microfluidic device for protein crystallization using an evaporation-based crystallization technique[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY,2012,45(2):53-60
APA Yu, Yong.,Wang, Xuan.,Oberthuer, Dominik.,Meyer, Arne.,Perbandt, Markus.,...&于泳.(2012).Design and application of a microfluidic device for protein crystallization using an evaporation-based crystallization technique.JOURNAL OF APPLIED CRYSTALLOGRAPHY,45(2),53-60.
MLA Yu, Yong,et al."Design and application of a microfluidic device for protein crystallization using an evaporation-based crystallization technique".JOURNAL OF APPLIED CRYSTALLOGRAPHY 45.2(2012):53-60.
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