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Title: Multi-patterned dynamics of mitochondrial fission and fusion in a living cell
Author: Wang SQ(王世骐); Xiao WM; Dan SC(单思聪); Jiang CS; Chen M; Zhang Y(章燕); Lv SQ(吕守芹); Chen J(陈娟); Zhang CM; Chen Quan; Long M(龙勉)
Source: PLOS ONE
Issued Date: 2012-05-23
Volume: 7, Issue:5, Pages:e19879
Abstract: Mitochondria are highly-dynamic organelles, but it is challenging to monitor quantitatively their dynamics in a living cell. Here we developed a novel approach to determine the global occurrence of mitochondrial fission and fusion events in living human epithelial cells (Hela) and mouse embryonic fibroblast cells (MEF). Distinct patterns of sequential events including fusion followed by fission (Fu-Fi), the so-called "kiss and run'' model previously described, fission followed by fusion (Fi-Fu), fusion followed by fusion (Fu-Fu), and fission followed by fission (Fi-Fi) were observed concurrently. The paired events appeared in high frequencies with short lifetimes and large sizes of individual mitochondria, as compared to those for unpaired events. The high frequencies of paired events were found to be biologically significant. The presence of membrane uncoupler CCCP enhanced the frequency of paired events (from both Fu-Fi and Fi-Fu patterns) with a reduced mitochondrial size. Knock-out of mitofusin protein Mfn1 increased the frequency of fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. These results indicated that the paired events were dominant but unpaired events were not negligible, which provided a new insight into mitochondrial dynamics. In addition to kiss and run model of action, our data suggest that, from a global visualization over an entire cell, multiple patterns of action appeared in mitochondrial fusion and fission.
Keyword: Mammalian-Cells ; Yeast ; Microtubules ; Segregation ; Morphology ; Apoptosis ; Transport ; Movement
Language: 英语
Indexed Type: SCI
Corresponding Author: Wang, SQ ; Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China.
Correspondent Email: chenq@ioz.ac.cn;mlong@imech.ac.cn
Related URLs: 查看原文
WOS ID: WOS:000305335800001
ISSN: 1932-6203
Rank: [Wang, Shiqi;Shan, Sicong; Zhang, Yan; Lu, Shouqin; Chen, Juan; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China; [Wang, Shiqi; Shan, Sicong; Zhang, Yan; Lu, Shouqin; Chen, Juan; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100080, Peoples R China; [Xiao, Weiming; Jiang, Chunsun; Chen, Ming; Chen, Quan] Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Beijing, Peoples R China; [Xiao, Weiming; Zhang, Chuanmao] Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China; [Xiao, Weiming; Zhang, Chuanmao] Peking Univ, Natl Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China; [Wang, Shiqi] Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Beijing, Peoples R China
Subject: 交叉与边缘领域的力学::生物力学
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类/Q1
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/46593
Appears in Collections:国家微重力实验室_期刊论文

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王世骐;Xiao WM;单思聪;Jiang CS;Chen M;章燕;吕守芹;陈娟;Zhang CM;Chen Quan;龙勉.Multi-patterned dynamics of mitochondrial fission and fusion in a living cell,PLOS ONE,2012,7(5):e19879
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