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Multi-patterned dynamics of mitochondrial fission and fusion in a living cell
Wang SQ(王世骐); Xiao WM; Dan SC(单思聪); Jiang CS; Chen M; Zhang Y(章燕); Lv SQ(吕守芹); Chen J(陈娟); Zhang CM; Chen Quan; Long M(龙勉); Wang, SQ; Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China.
Source PublicationPLOS ONE
2012-05-23
Volume7Issue:5Pages:e19879
ISSN1932-6203
AbstractMitochondria are highly-dynamic organelles, but it is challenging to monitor quantitatively their dynamics in a living cell. Here we developed a novel approach to determine the global occurrence of mitochondrial fission and fusion events in living human epithelial cells (Hela) and mouse embryonic fibroblast cells (MEF). Distinct patterns of sequential events including fusion followed by fission (Fu-Fi), the so-called "kiss and run'' model previously described, fission followed by fusion (Fi-Fu), fusion followed by fusion (Fu-Fu), and fission followed by fission (Fi-Fi) were observed concurrently. The paired events appeared in high frequencies with short lifetimes and large sizes of individual mitochondria, as compared to those for unpaired events. The high frequencies of paired events were found to be biologically significant. The presence of membrane uncoupler CCCP enhanced the frequency of paired events (from both Fu-Fi and Fi-Fu patterns) with a reduced mitochondrial size. Knock-out of mitofusin protein Mfn1 increased the frequency of fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. These results indicated that the paired events were dominant but unpaired events were not negligible, which provided a new insight into mitochondrial dynamics. In addition to kiss and run model of action, our data suggest that, from a global visualization over an entire cell, multiple patterns of action appeared in mitochondrial fusion and fission.
KeywordMammalian-cells Yeast Microtubules Segregation Morphology Apoptosis Transport Movement
Subject Area生物力学
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Indexed BySCI
Language英语
WOS IDWOS:000305335800001
Funding OrganizationThis work was supported by Chinese Academy of Sciences grant KJCX2-YW-L08, National Natural Science (http://english.cas.cn/), Foundation of China grants 30730032 and 30730093 (http://www.nsfc.gov.cn/Portal0/default106.htm), National Key Basic Research Foundation of China grant 2006CB910303 (http://www.most.gov.cn/eng/programmes1/200610/t20061009_36223.htm), and National High Technology Research and Development Program of China grant 2007AA02Z306 (http://www.most.gov.cn/eng/programmes1/index.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类/Q1
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/46593
Collection国家微重力实验室
Corresponding AuthorWang, SQ; Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China.
Recommended Citation
GB/T 7714
Wang SQ,Xiao WM,Dan SC,et al. Multi-patterned dynamics of mitochondrial fission and fusion in a living cell[J]. PLOS ONE,2012,7(5):e19879.
APA 王世骐.,Xiao WM.,单思聪.,Jiang CS.,Chen M.,...&Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China..(2012).Multi-patterned dynamics of mitochondrial fission and fusion in a living cell.PLOS ONE,7(5),e19879.
MLA 王世骐,et al."Multi-patterned dynamics of mitochondrial fission and fusion in a living cell".PLOS ONE 7.5(2012):e19879.
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