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Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing
Niu Y(牛宇); Matos AI; Abrantes LM; Viana AS; Jin G(靳刚); Viana A.S.
Source PublicationLANGMUIR
2012-12-21
Volume28Issue:51Pages:17718-17725
ISSN0743-7463
AbstractCarbon disulfide (CS2) can spontaneously react with amine groups to form dithiocarbamates on gold surface, providing the possibility to immobilize some compounds with primary or secondary amine groups in one step. Using this principle, an immunosensor interface prepared for immunoglobulin G (IgG) sensing surface toward anti-IgG has been fabricated for the first time by simply immersing gold slides into a mixed aqueous solution of CS2 and protein A, followed by incubation in immunoglobulin G solution. The reaction between CS2 and protein A has been followed by UV-vis spectroscopy, whereas cyclic voltammetry has been employed in the characterization of the modified gold surface with CS2 and protein A, both methods indicating that protein A immobilization is implemented by CS2. Conventional ellipsometry, atomic force microscopy (AFM), as well as surface plasmon resonance (SPR) have been used to evaluate the specific binding of protein A with IgG and IgG with anti-IgG, revealing that IgG is specifically captured to form the biosensing interface, maintaining its bioactivity. Compared to direct adsorption of IgG on the gold surface, the IgG sensing surface constructed of CS2 and protein A is far more sensitive to capture anti-IgG as its target molecule. In addition, the modified surface is proven to have good capability to inhibit nonspecific adsorption, as supported by control experiments using lysozyme and BSA. To conclude, antibody immobilization using this one-step method has potential as a simple and convenient surface modification approach for immunosensor development.; Ial as a simple and convenient surface modification approach for immunosensor development.
KeywordAdsorption Atomic Force Microscopy Carbon Disulfide Cyclic Voltammetry Gold Immunosensors Organic Compounds Surface Plasmon Resonance Ultraviolet Visible Spectroscopy
Subject Area交叉与边缘领域的力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000315326300017
DepartmentNML纳米生物光学
Classification一类
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/46783
Collection国家微重力实验室
Corresponding AuthorViana A.S.
Recommended Citation
GB/T 7714
Niu Y,Matos AI,Abrantes LM,et al. Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing[J]. LANGMUIR,2012,28(51):17718-17725.
APA Niu Y,Matos AI,Abrantes LM,Viana AS,Jin G,&Viana A.S..(2012).Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing.LANGMUIR,28(51),17718-17725.
MLA Niu Y,et al."Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing".LANGMUIR 28.51(2012):17718-17725.
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