Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing

IMECH-IR > 微重力重点实验室

	Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing
	Niu Y(牛宇); Matos AI; Abrantes LM; Viana AS; Jin G(靳刚); Viana A.S.
发表期刊	LANGMUIR
	2012-12-21
卷号	28 期号:51 页码:17718-17725
ISSN	0743-7463
摘要	Carbon disulfide (CS2) can spontaneously react with amine groups to form dithiocarbamates on gold surface, providing the possibility to immobilize some compounds with primary or secondary amine groups in one step. Using this principle, an immunosensor interface prepared for immunoglobulin G (IgG) sensing surface toward anti-IgG has been fabricated for the first time by simply immersing gold slides into a mixed aqueous solution of CS2 and protein A, followed by incubation in immunoglobulin G solution. The reaction between CS2 and protein A has been followed by UV-vis spectroscopy, whereas cyclic voltammetry has been employed in the characterization of the modified gold surface with CS2 and protein A, both methods indicating that protein A immobilization is implemented by CS2. Conventional ellipsometry, atomic force microscopy (AFM), as well as surface plasmon resonance (SPR) have been used to evaluate the specific binding of protein A with IgG and IgG with anti-IgG, revealing that IgG is specifically captured to form the biosensing interface, maintaining its bioactivity. Compared to direct adsorption of IgG on the gold surface, the IgG sensing surface constructed of CS2 and protein A is far more sensitive to capture anti-IgG as its target molecule. In addition, the modified surface is proven to have good capability to inhibit nonspecific adsorption, as supported by control experiments using lysozyme and BSA. To conclude, antibody immobilization using this one-step method has potential as a simple and convenient surface modification approach for immunosensor development.; Ial as a simple and convenient surface modification approach for immunosensor development.
关键词	Adsorption Atomic Force Microscopy Carbon Disulfide Cyclic Voltammetry Gold Immunosensors Organic Compounds Surface Plasmon Resonance Ultraviolet Visible Spectroscopy
学科领域	交叉与边缘领域的力学
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000315326300017
课题组名称	NML纳米生物光学
论文分区	一类
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/46783
专题	微重力重点实验室
通讯作者	Viana A.S.
推荐引用方式 GB/T 7714	Niu Y,Matos AI,Abrantes LM,et al. Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing[J]. LANGMUIR,2012,28,51,:17718-17725.
APA	Niu Y,Matos AI,Abrantes LM,Viana AS,Jin G,&Viana A.S..(2012).Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing.LANGMUIR,28(51),17718-17725.
MLA	Niu Y,et al."Antibody oriented immobilization on gold using the reaction between carbon disulfide and amine groups and its application in immunosensing".LANGMUIR 28.51(2012):17718-17725.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
SCI-J2012-234.pdf（3005KB）			开放获取	--	浏览请求全文