Five days of head-down-tilt bed rest induces noninflammatory shedding of L-selectin

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	Five days of head-down-tilt bed rest induces noninflammatory shedding of L-selectin
	Feuerecker M; Feuerecker B; Matzel S; Long M(龙勉); Strewe C; Kaufmann I; Hoerl M; Schelling G; Rehm M; Chouker A; Chouker, A (reprint author), Univ Munich, Dept Anaesthesiol, Marchioninistr 15, D-81377 Munich, Germany.
发表期刊	JOURNAL OF APPLIED PHYSIOLOGY
	2013-07-01
卷号	115 期号:2 页码:235-242
ISSN	8750-7587
摘要	Head-down-tilt bed rest (HDTBR) is a popular model, simulating alterations of gravitation during space missions. The aim of this study was to obtain a better insight into the complexly orchestrated regulations of HDTBR-induced immunological responses, hypothesizing that artificial gravity can mitigate these HDTBR-related physiological effects. This crossover-designed 5 days of HDTBR study included three protocols with no, or daily 30 min of centrifugation or 6 X 5 min of centrifugation. Twelve healthy, male participants donated blood pre-HDTBR, post-HDTBR, and twice during HDTBR. Cellular immune changes were assessed either by enumerative and immune cell phenotyping assays or by functional testing of responses to either recall antigens or receptor-dependent activation by chemotactic agents N-formyl-methionyl-leucyl-phenylalanine (fMLP) and with TNF-alpha. The expression of the adhesion molecule L-selectin (CD62L) on the surface of granulocytes and its shedding into plasma samples were measured. In parallel, other humoral factor, such as interleukin-6 and interleukin-8, parameters of endothelial damage (glycocalyx) were determined. Hematocrit and hemoglobin were significantly increased during HDTBR. Although immune functional tests did not indicate a change in the immune performance, the expression of CD62L on resting granulocytes was significantly shed by 50% during HDTBR. Although the latter is normally associated to an activation of inflammatory innate immune responses and during interaction of granulocytes with the endothelium, CD62L shedding was, however, not related either to a systemic inflammatory alteration or to shedding of the endothelial glycocalyx during bed rest. This suggests a noninflammatory or "mechanical" shedding related to fluid shifts during head-down intervention and not to an acute inflammatory process.
关键词	Cd62l Shedding Immunological Changes Short-term Bed Rest
学科领域	交叉与边缘领域的力学
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000321819000012
项目资助者	European Space Agency; German National Space Program [50WB0719/WB0919]
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	二类/Q1
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/47359
专题	微重力重点实验室
通讯作者	Chouker, A (reprint author), Univ Munich, Dept Anaesthesiol, Marchioninistr 15, D-81377 Munich, Germany.
推荐引用方式 GB/T 7714	Feuerecker M,Feuerecker B,Matzel S,et al. Five days of head-down-tilt bed rest induces noninflammatory shedding of L-selectin[J]. JOURNAL OF APPLIED PHYSIOLOGY,2013,115,2,:235-242.
APA	Feuerecker M.,Feuerecker B.,Matzel S.,Long M.,Strewe C.,...&Chouker, A .(2013).Five days of head-down-tilt bed rest induces noninflammatory shedding of L-selectin.JOURNAL OF APPLIED PHYSIOLOGY,115(2),235-242.
MLA	Feuerecker M,et al."Five days of head-down-tilt bed rest induces noninflammatory shedding of L-selectin".JOURNAL OF APPLIED PHYSIOLOGY 115.2(2013):235-242.

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