Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading

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	Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading
	Li JJ; Han D; Zhao YP(赵亚溥); Zhao, YP (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China.
发表期刊	Scientific Reports
	2014-01-28
卷号	4 页码:3910
ISSN	2045-2322
摘要	To describe detailed behaviour of cell spreading under the influence of substrate stiffness, A549 cells cultured on the surfaces of polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) with bulk rigidities ranging from 0.1 kPa to 40 kPa were in situ observed. The spreading behaviour of cells on PAAm presented a positive correlation between spreading speed and substrate stiffness. After computing the deformations of PAAm gels and collagen, the bulk stiffness of PAAm, rather than matrix tethering, determined the cell behaviour. On the other hand, spreading behaviour of the cells was unaffected by varying the bulk stiffness of PDMS. Based on simulation analyses, the elasticity of silica-like layer induced by UV radiation on PDMS surface dominated cell-substrate interaction, rather than the bulk stiffness of the material, indicating that it is the interfacial stiffness that mainly guided the cell spreading. And then the kinetics of cell spreading was for the first time modeled based on absolute rate theory.
学科领域	Science & Technology - Other Topics
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000331152700004
项目资助者	This work was jointly supported by the National Natural Science Foundation of China (NSFC, Grant No. 11072244), the Key Research Program of the Chinese Academy of Sciences (Grant No. KJZD-EW-M01), the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. Y2010031) and the project of 973 in Ministry of Science and Technology of China (Grant No. 2012CB933800).
课题组名称	LNM纳/微系统力学与物理力学
论文分区	一类
引用统计	被引频次：82[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/48779
专题	非线性力学国家重点实验室
通讯作者	Zhao, YP (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Li JJ,Han D,Zhao YP,et al. Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading[J]. Scientific Reports,2014,4:3910.
APA	Li JJ,Han D,Zhao YP,&Zhao, YP .(2014).Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading.Scientific Reports,4,3910.
MLA	Li JJ,et al."Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading".Scientific Reports 4(2014):3910.

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