Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry

doi:10.1016/j.tsf.2014.02.098

IMECH-IR > 微重力重点实验室

	Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
	Kang TF(康腾飞); Niu Y(牛宇); Jin G(靳刚); Jin, G (reprint author), 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
发表期刊	Thin Solid Films
	2014-11-28
卷号	571 页码:463-467
ISSN	0040-6090
摘要	Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However, it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry (TIRIE) is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme (LZM), a typical example of weak affinity interactions, has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10(-5) M, which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Biosensor Total Internal Reflection Imaging Ellipsometry Weak Affinity Interaction Tris Lysozyme
学科领域	Materials Science ; Physics
DOI	10.1016/j.tsf.2014.02.098
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000346055200022
关键词[WOS]	PLASMON RESONANCE BIOSENSOR ; POLARIZATION ; RECOGNITION ; AFFINITIES ; ANTIGEN
WOS研究方向	Materials Science ; Physics
WOS类目	Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
课题组名称	NML纳米生物光学
论文分区	二类/Q2
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/49492
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Kang TF,Niu Y,Jin G,et al. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[J]. Thin Solid Films,2014,571:463-467.
APA	Kang TF,Niu Y,Jin G,&Jin, G .(2014).Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry.Thin Solid Films,571,463-467.
MLA	Kang TF,et al."Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry".Thin Solid Films 571(2014):463-467.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
IMCAS-J2014-350.pdf（690KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载