Kinetic analysis of the weak affinity interaction between tris and lysozyme

doi:10.1016/j.bbrc.2015.01.044

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	Kinetic analysis of the weak affinity interaction between tris and lysozyme
	Kang TF(康腾飞); Hao WX(郝文欣); Niu Y(牛宇); Luo ZR(罗子人); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2015-02-20
卷号	457 期号:4 页码:659-663
ISSN	0006-291X
摘要	The biosensor based on total internal reflection imaging ellipsometry (TIRIE), regarded as an automotive real-time research approach for biomolecular interaction, is introduced to analyze the kinetic process of the weak interaction between tris and lysozyme. The experiment is performed by delivering lysozyme solution diluted to different concentrations to the biosensor substrate interface immobilized with tris. By applying pseudo-first-order interaction kinetics model, we are able to obtain the kinetic parameters from fitting experimental data. The calculated association rate constant and dissociation rate constant of tris and lysozyme interaction are in 10(-2) mol(-1) s(-1) and 10(3) s(-1) magnitude, respectively. To further improve TIRIE's ability for kinetically characterizing biomolecular interaction, a theoretical method to deduce associate rate constant before experiment is proposed. (C) 2015 Elsevier Inc. All rights reserved.
关键词	Kinetic Analysis Weak Affinity Interaction Tirie Biosensor Pseudo-first-order Interaction Kinetics Association Rate Constant
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbrc.2015.01.044
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000350838800028
关键词[WOS]	REFLECTION IMAGING ELLIPSOMETRY ; BIOMOLECULAR INTERACTIONS ; PROTEIN INTERACTIONS ; BIOSENSOR ; ADSORPTION ; MODEL
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
项目资助者	National Basic Research Program of China [2015CB352100] ; National Natural Science Foundation of China [21305147, 81472941] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Seventh Sino-Portugal Scientific and Technological Cooperation
课题组名称	NML纳米生物光学
论文分区	Q3
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/49926
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Kang TF,Hao WX,Niu Y,et al. Kinetic analysis of the weak affinity interaction between tris and lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457,4,:659-663.
APA	Kang TF,Hao WX,Niu Y,Luo ZR,Jin G,&Jin, G .(2015).Kinetic analysis of the weak affinity interaction between tris and lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,457(4),659-663.
MLA	Kang TF,et al."Kinetic analysis of the weak affinity interaction between tris and lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 457.4(2015):659-663.

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