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Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
Kang TF(康腾飞); Niu Y(牛宇); Jin G(靳刚); gajin@imech.ac.cn
Source PublicationTHIN SOLID FILMS
2014
Conference Name6th International Conference on Spectroscopic Ellipsometry
Conference DateMAY 26-31 2013
Conference PlaceKyoto JAPAN
AbstractWeak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme a typical example of weak affinity interactions has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10 M which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. 2014 Elsevier B.V. All rights reserved.
KeywordBiosensor Total Internal Reflection Imaging Ellipsometry Weak Affinity Interaction Tris Lysozyme
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Indexed BySCI ; CPCI
Language英语
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/55365
Collection国家微重力实验室
Corresponding Authorgajin@imech.ac.cn
Recommended Citation
GB/T 7714
Kang TF,Niu Y,Jin G,et al. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[C]THIN SOLID FILMS,2014.
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