Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry

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	Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
	Kang TF(康腾飞); Niu Y(牛宇); Jin G(靳刚); gajin@imech.ac.cn
会议录名称	THIN SOLID FILMS
	2014
会议名称	6th International Conference on Spectroscopic Ellipsometry
会议日期	MAY 26-31 2013
会议地点	Kyoto JAPAN
摘要	Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme a typical example of weak affinity interactions has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10 M which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. 2014 Elsevier B.V. All rights reserved.
关键词	Biosensor Total Internal Reflection Imaging Ellipsometry Weak Affinity Interaction Tris Lysozyme
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收录类别	SCI ; CPCI
语种	英语
文献类型	会议论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/55365
专题	微重力重点实验室
通讯作者	gajin@imech.ac.cn
推荐引用方式 GB/T 7714	Kang TF,Niu Y,Jin G,et al. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[C]THIN SOLID FILMS,2014.

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