Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer

doi:10.1016/j.msec.2011.05.018

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	Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer
	Huang CH; Li JX; Tang Y; Wang CX; Hou CJ; Huo DQ; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
发表期刊	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2011
卷号	31 期号:7 页码:1609-1613
ISSN	0928-4931
摘要	In this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 x 10(3) CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1,2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Biosensor Imaging Ellipsometry Riemerella Anatipestifer Detection
DOI	10.1016/j.msec.2011.05.018
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000295953900051
关键词[WOS]	PASTEURELLA-ANATIPESTIFER ; ESCHERICHIA-COLI ; IMMUNOSENSOR ; DUCKS ; VISUALIZATION ; INFECTION
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
项目资助者	Natural Science Foundation [20845003, 10804083] ; Ministry of Science and Technology of China [2008AA02Z419] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Natural Science Foundation of Chongqing [CSTC2008 BB1245] ; Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences
课题组名称	NML纳米生物光学
论文分区	一类
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/58582
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Huang CH,Li JX,Tang Y,et al. Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2011,31,7,:1609-1613.
APA	Huang CH.,Li JX.,Tang Y.,Wang CX.,Hou CJ.,...&Jin, G .(2011).Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,31(7),1609-1613.
MLA	Huang CH,et al."Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 31.7(2011):1609-1613.

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