IMECH-IR  > 国家微重力实验室
A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry
Huang CH; Chen YY(陈艳艳); Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China.
Source PublicationANNALS OF BIOMEDICAL ENGINEERING
2011
Volume39Issue:1Pages:185-192
ISSN0090-6964
AbstractCancer markers can offer much more information for developing or developed cancers. Simple and short-time assay of cancer biomarker is essential for clinic diagnosis and management. In this study, a one-step immunoassay for carbohydrate antigen 19-9 (CA19-9) by biosensor based on imaging ellipsometry (BIE) is described and its analysis performances are evaluated. Anti-CA19-9 monoclonal antibody as ligand covalently immobilized on carboxyl-modified silicon substrate and its immobilization concentration concurrently screened. Then, bovine serum albumin (BSA) is used to block for acquisition of sensing layer. The detection linear range is from 15.0 to 120.0 U/mL with a minimum limit of 10.0 U/mL (S/N = 3). The intra-slide and inter-slide reproducibility, expressed as coefficient variation (CV%), are 9.9, 5.9, 5.4% and 9.6, 7.6, 5.3% for 31.9, 57.4, and 99.2 U/mL of CA19-9, respectively. Accuracy determined by spiked-recovery is among 95.1-106.7%. Specificity expressed as cross-reaction rate are all below 5.8% evaluated by three analogous biomarkers alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and carbohydrate antigen 242 (CA242). Application of BIE to 25 clinic samples demonstrated that the results are in high correlate with electrochemiluminescent immunoassay (ECLIA, R-2 = 0.997). The proposed immunoassay has the potential for clinic application.
KeywordBiosensor Imaging Ellipsometry Carbohydrate Antigen 19-9 Immunoassay
DOI10.1007/s10439-010-0174-4
Indexed BySCI ; EI
Language英语
WOS IDWOS:000287213100017
WOS KeywordOPTICAL PROTEIN-CHIP ; SPECTROSCOPIC ELLIPSOMETRY ; COLORECTAL-CANCER ; IMMOBILIZATION ; IMMUNOSENSOR ; MICROARRAYS ; SURFACE ; VISUALIZATION ; LAYERS ; ASSAY
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
Funding OrganizationMinistry of Science and Technology of China [2008AA02Z419] ; Natural Science Foundation of China [10804083, 20845003] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences
DepartmentNML纳米生物光学
Classification二类/Q2
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58591
Collection国家微重力实验室
Corresponding AuthorChen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China.
Recommended Citation
GB/T 7714
Huang CH,Chen YY,Jin G,et al. A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry[J]. ANNALS OF BIOMEDICAL ENGINEERING,2011,39(1):185-192.
APA Huang CH,陈艳艳,靳刚,&Chen, YY .(2011).A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry.ANNALS OF BIOMEDICAL ENGINEERING,39(1),185-192.
MLA Huang CH,et al."A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry".ANNALS OF BIOMEDICAL ENGINEERING 39.1(2011):185-192.
Files in This Item: Download All
File Name/Size DocType Version Access License
JourArSuppl2016-023.(611KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang CH]'s Articles
[陈艳艳]'s Articles
[靳刚]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang CH]'s Articles
[陈艳艳]'s Articles
[靳刚]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang CH]'s Articles
[陈艳艳]'s Articles
[靳刚]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: JourArSuppl2016-023.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.