| A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry |
| Huang CH; Chen YY(陈艳艳) ; Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China.
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| 发表期刊 | ANNALS OF BIOMEDICAL ENGINEERING
 |
| 2011
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| 卷号 | 39期号:1页码:185-192 |
| ISSN | 0090-6964
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| 摘要 | Cancer markers can offer much more information for developing or developed cancers. Simple and short-time assay of cancer biomarker is essential for clinic diagnosis and management. In this study, a one-step immunoassay for carbohydrate antigen 19-9 (CA19-9) by biosensor based on imaging ellipsometry (BIE) is described and its analysis performances are evaluated. Anti-CA19-9 monoclonal antibody as ligand covalently immobilized on carboxyl-modified silicon substrate and its immobilization concentration concurrently screened. Then, bovine serum albumin (BSA) is used to block for acquisition of sensing layer. The detection linear range is from 15.0 to 120.0 U/mL with a minimum limit of 10.0 U/mL (S/N = 3). The intra-slide and inter-slide reproducibility, expressed as coefficient variation (CV%), are 9.9, 5.9, 5.4% and 9.6, 7.6, 5.3% for 31.9, 57.4, and 99.2 U/mL of CA19-9, respectively. Accuracy determined by spiked-recovery is among 95.1-106.7%. Specificity expressed as cross-reaction rate are all below 5.8% evaluated by three analogous biomarkers alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and carbohydrate antigen 242 (CA242). Application of BIE to 25 clinic samples demonstrated that the results are in high correlate with electrochemiluminescent immunoassay (ECLIA, R-2 = 0.997). The proposed immunoassay has the potential for clinic application. |
| 关键词 | Biosensor
Imaging Ellipsometry
Carbohydrate Antigen 19-9
Immunoassay
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| DOI | 10.1007/s10439-010-0174-4
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| 收录类别 | SCI
; EI
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| 语种 | 英语
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| WOS记录号 | WOS:000287213100017
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| 关键词[WOS] | OPTICAL PROTEIN-CHIP
; SPECTROSCOPIC ELLIPSOMETRY
; COLORECTAL-CANCER
; IMMOBILIZATION
; IMMUNOSENSOR
; MICROARRAYS
; SURFACE
; VISUALIZATION
; LAYERS
; ASSAY
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| WOS研究方向 | Engineering
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| WOS类目 | Engineering, Biomedical
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| 项目资助者 | Ministry of Science and Technology of China [2008AA02Z419]
; Natural Science Foundation of China [10804083, 20845003]
; Chinese Academy of Sciences [KJCX2.YW.M04]
; Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences
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| 课题组名称 | NML纳米生物光学
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| 论文分区 | 二类/Q2
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/58591
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| 专题 | 微重力重点实验室
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| 通讯作者 | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China. |
推荐引用方式
GB/T 7714 |
Huang CH,Chen YY,Jin G,et al. A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry[J]. ANNALS OF BIOMEDICAL ENGINEERING,2011,39,1,:185-192.
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| APA |
Huang CH,陈艳艳,靳刚,&Chen, YY .(2011).A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry.ANNALS OF BIOMEDICAL ENGINEERING,39(1),185-192.
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| MLA |
Huang CH,et al."A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry".ANNALS OF BIOMEDICAL ENGINEERING 39.1(2011):185-192.
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