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Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique
Yang H; Tong CF; Fu CL; Xu YH; Liu XF; Chen Q; Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
Source PublicationJOURNAL OF IMMUNOLOGICAL METHODS
2016-01
Volume428Pages:20-29
ISSN0022-1759
AbstractAdhesion between two nucleated cells undergoes specific significances in immune responses and tumor metastasis since cellular adhesive molecules usually express on two apposed cell membranes. However, quantification of the interactions between two nucleated cells is still challenging in microvasculature. Here distinct cell systems were used, including three types of human cells (Jurkat cell or PMN vs. MDA-MB-231 cell) and two kinds of murine native cells (PMN vs. liver sinusoidal endothelial cell). Cell movement, compression to, and relaxation from the counterpart cell were quantified using an in-house developed gas-driven micropipette aspiration technique (GDMAT). This assay is robust to quantify this process since cell movement and contact inside a pipette are independent of the repeated test cycles. Measured approaching or retraction velocity follows well a normal distribution, which is independent on the cycle period. Contact area or duration also fits a Gaussian distribution and moreover contact duration is linearly correlated with the cycle period. Cell movement is positively related to gas flux but negatively associated to medium viscosity. Cell adhesion tends to reach an equilibrium state with increase of cycle period or contact duration. These results further the understanding in the dynamics of cell movement and contact in microvasculature. (C) 2015 Elsevier B.V. All rights reserved.
KeywordNucleated Cells Movement Contact Adhesion Pipette
DOI10.1016/j.jim.2015.11.007
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000369451600003
WOS KeywordTHERMAL BARRIER COATINGS ; FRACTURE CHARACTERISTICS ; MECHANICAL-PROPERTIES ; ACOUSTIC-EMISSION ; EVOLUTION ; THICKNESS ; TENSILE ; ENERGY ; TESTS
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemical Research Methods ; Immunology
Funding OrganizationThis work was supported by the National Natural Science Foundation of China grants 31230027 and 31110103918, National Key Basic Research Foundation of China grant 2011CB710904, and Strategic Priority Research Program of Chinese Academy of Sciences grant XDA01030102.
DepartmentNML分子-细胞生物力学与空间生命科学
ClassificationQ3
RankingTrue
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58683
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Yang H,Tong CF,Fu CL,et al. Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2016,428:20-29.
APA Yang H.,Tong CF.,Fu CL.,Xu YH.,Liu XF.,...&Long, M .(2016).Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique.JOURNAL OF IMMUNOLOGICAL METHODS,428,20-29.
MLA Yang H,et al."Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique".JOURNAL OF IMMUNOLOGICAL METHODS 428(2016):20-29.
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