Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique

doi:10.1016/j.jim.2015.11.007

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	Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique
	Yang H; Tong CF; Fu CL; Xu YH; Liu XF; Chen Q; Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
发表期刊	JOURNAL OF IMMUNOLOGICAL METHODS
	2016-01
卷号	428 页码:20-29
ISSN	0022-1759
摘要	Adhesion between two nucleated cells undergoes specific significances in immune responses and tumor metastasis since cellular adhesive molecules usually express on two apposed cell membranes. However, quantification of the interactions between two nucleated cells is still challenging in microvasculature. Here distinct cell systems were used, including three types of human cells (Jurkat cell or PMN vs. MDA-MB-231 cell) and two kinds of murine native cells (PMN vs. liver sinusoidal endothelial cell). Cell movement, compression to, and relaxation from the counterpart cell were quantified using an in-house developed gas-driven micropipette aspiration technique (GDMAT). This assay is robust to quantify this process since cell movement and contact inside a pipette are independent of the repeated test cycles. Measured approaching or retraction velocity follows well a normal distribution, which is independent on the cycle period. Contact area or duration also fits a Gaussian distribution and moreover contact duration is linearly correlated with the cycle period. Cell movement is positively related to gas flux but negatively associated to medium viscosity. Cell adhesion tends to reach an equilibrium state with increase of cycle period or contact duration. These results further the understanding in the dynamics of cell movement and contact in microvasculature. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Nucleated Cells Movement Contact Adhesion Pipette
DOI	10.1016/j.jim.2015.11.007
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000369451600003
关键词[WOS]	THERMAL BARRIER COATINGS ; FRACTURE CHARACTERISTICS ; MECHANICAL-PROPERTIES ; ACOUSTIC-EMISSION ; EVOLUTION ; THICKNESS ; TENSILE ; ENERGY ; TESTS
WOS研究方向	Biochemistry & Molecular Biology ; Immunology
WOS类目	Biochemical Research Methods ; Immunology
项目资助者	This work was supported by the National Natural Science Foundation of China grants 31230027 and 31110103918, National Key Basic Research Foundation of China grant 2011CB710904, and Strategic Priority Research Program of Chinese Academy of Sciences grant XDA01030102.
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	Q3
力学所作者排名	True
引用统计
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/58683
专题	微重力重点实验室
通讯作者	Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Yang H,Tong CF,Fu CL,et al. Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2016,428:20-29.
APA	Yang H.,Tong CF.,Fu CL.,Xu YH.,Liu XF.,...&Long, M .(2016).Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique.JOURNAL OF IMMUNOLOGICAL METHODS,428,20-29.
MLA	Yang H,et al."Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique".JOURNAL OF IMMUNOLOGICAL METHODS 428(2016):20-29.

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