Knowledge Management System of Institue of Mechanics, CAS
| Immune-microassay with optical proteinchip for protein detection | |
Jin G(靳刚) ; Wang ZH; Qi C; Zhao ZY; Chen S(陈涉); Meng YH; Ying PQ; Xia LH; Wan LJ; Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100080, Peoples R China.
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| Source Publication | PROCEEDINGS OF THE 25TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-4: A NEW BEGINNING FOR HUMAN HEALTH |
| 2003 | |
| Conference Name | 25th Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society |
| Conference Date | SEP 17-21, 2003 |
| Conference Place | Cancun, MEXICO |
| Abstract | An optical proteinchip upon antigen-antibody affinity and the chip analysis system have been used in immune analysis. The chip is processed with chip design, surface patterning, surface modification, ligand immobilization to form a microarray with many immune probes - one kind of antigen (or antibody) occupying one unit in the microarray and many such units in matrix. In micro-reactor of bio-molecule interaction, when ligand met its receptor, they would bind into bio-specific complex, which resulted in the thickness of the probe surface layer (surface concentration of antigen-antibody) increasing. The variation of protein pattern on the chip surface corresponding to the ligand immobilization and protein interactions was screened with optical imaging ellipsometry. A non-labeling micro-assay for protein detection was realized with antigen-antibody affinity and non-disturbance optical sampling, which was available for the direct test of crude samples, such as serum, etc. Some application results in immune analysis were presented also. |
| WOS ID | WOS:000189395300931 |
| ISBN | 0-7803-7789-3 |
| Indexed By | CPCI-S ; EI |
| Language | 英语 |
| Citation statistics | |
| Document Type | 会议论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/58930 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Jin G,Wang ZH,Qi C,et al. Immune-microassay with optical proteinchip for protein detection[C]PROCEEDINGS OF THE 25TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-4: A NEW BEGINNING FOR HUMAN HEALTH,2003. |
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| File Name/Size | DocType | Version | Access | License | ||
| CPCI-B027.pdf（211KB） | 会议论文 | 开放获取 | CC BY-NC-SA | View Download | ||
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