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Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration
Gong YX; Zhang Y(章燕); Feng SL; Liu XF; Lv SQ(吕守芹); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.; Long, M (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
Source PublicationFASEB JOURNAL
2017
Volume31Issue:1Pages:212-+
ISSN0892-6638
Abstract

Leukocyte transendothelial migrationis a key step in their recruitment to sites of inflammation. However, synergic regulation of endothelium-expressed selectins on leukocyte transmigration remains unclear. In this study, an in vitro model was developed to investigate the dynamic contributions of P- and E-selectin to polymorphonuclear neutrophil (PMN) transmigration under static conditions. Human umbilical vein endothelial cells (HUVECs) were treated with LPS for 4 or 12 h to induce different expression of selectins and intercellular adhesion molecule (ICAM)-1. PMN transmigration was increased significantly by LPS stimulation, which was higher on 4-h than on 12-h LPS treated HUVECs. Blocking and competitive tests indicated that P-selectin engages PSGL-1 to activate beta(2)-integrin and initiate PMN transmigration within the first 15 min, whereas E-selectin engages CD44 to influence PMN transmigration after 15 min. P-and E-selectin-induced beta(2)-integrin activation is likely conducted through the spleen tyrosine kinase signaling pathway. Complicated complementary and competitive mechanisms are involved in the interaction of P-/E-selectins and their ligands to promote PMN transmigration. These results provide direct evidence of the distinct and dynamic contribution of P- and E-selectins inmediating PMN transmigration and give new insight into PMN interaction with the vessel wall.-Gong, Y., Zhang, Y., Feng, S., Liu, X., Lu, S., Long, M. Dynamic contributions of P-and E-selectins to beta(2)-integrin-induced neutrophil transmigration.

DOI10.1096/fj.201600398RRR
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Indexed BySCI
Language英语
WOS IDWOS:000392177600022
WOS KeywordPsgl-1 ; Cd44 ; Synergic Mechanisms
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biology ; Cell Biology
Funding OrganizationThis work was supported by National Natural Science Foundation of China Grants 31230027, 91539119 and 31270994 ; National Key Basic Research Foundation of China Grant 2011CB710904 ; Strategic Priority Research Program Grant XDA01030102 ; and National High Technology Research and Development Program of China Grant 2011AA020109. The authors declare no conflicts of interest.
DepartmentNML分子-细胞生物力学与空间生命科学
Classification一类
Ranking1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60110
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.; Long, M (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Gong YX,Zhang Y,Feng SL,et al. Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration[J]. FASEB JOURNAL,2017,31(1):212-+.
APA Gong YX.,Zhang Y.,Feng SL.,Liu XF.,Lv SQ.,...&Long, M .(2017).Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration.FASEB JOURNAL,31(1),212-+.
MLA Gong YX,et al."Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration".FASEB JOURNAL 31.1(2017):212-+.
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