IMECH-IR  > 力学所知识产出(1956-2008)
Silicon surface modification with supported phospholipids bilayer for biosensor based on imaging ellipsometry
Chen YY(陈艳艳); Wang ZH; Liu Y; Liang W; Chang WR; Jin G(靳刚)
Source PublicationIFMBE Proceedings
2008
Conference Name4th European Conference of the International Federation for Medical and Biological Engineering, ECIFMBE 2008
Conference DateNovember 23, 2008 - November 27, 2008
Conference PlaceAntwerp, Belgium
AbstractA method is presented for fabricating supported phospholipids bilayer on silicon surface for biosensor based on imaging ellipsometry. The lipid bilayer prepared by covalent immobilization of phosphatidylethanolamine layer on silicon surface followed by adsorption of PEG functionalized phospholipids layer, is highly protein resistant and stable, which can withstand multiple cycles of drying and rehydration, remaining intact in various buffers. Proteins covalently immobilized on the bilayer by the terminal p-nitrophenylcarbonyl or carboxyl group of PEG remain higher bioactivity than that immobilized on silicon surface directly. © 2009 Springer Berlin Heidelberg.
KeywordBi-layer Carboxyl Groups Covalent Immobilization Functionalized Imaging Ellipsometry Microfluidic Array Phosphatidylethanolamine Phospholipids Bilayer Silicon Surfaces
WOS IDWOS:000299998500551
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Indexed ByCPCI-S ; EI
Language英语
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Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60310
Collection力学所知识产出(1956-2008)
Recommended Citation
GB/T 7714
Chen YY,Wang ZH,Liu Y,et al. Silicon surface modification with supported phospholipids bilayer for biosensor based on imaging ellipsometry[C],2008.
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