Knowledge Management System of Institue of Mechanics, CAS
Silicon surface modification with supported phospholipids bilayer for biosensor based on imaging ellipsometry | |
Chen YY(陈艳艳); Wang ZH; Liu Y; Liang W; Chang WR; Jin G(靳刚) | |
会议录名称 | IFMBE Proceedings |
2008 | |
会议名称 | 4th European Conference of the International Federation for Medical and Biological Engineering, ECIFMBE 2008 |
会议日期 | November 23, 2008 - November 27, 2008 |
会议地点 | Antwerp, Belgium |
摘要 | A method is presented for fabricating supported phospholipids bilayer on silicon surface for biosensor based on imaging ellipsometry. The lipid bilayer prepared by covalent immobilization of phosphatidylethanolamine layer on silicon surface followed by adsorption of PEG functionalized phospholipids layer, is highly protein resistant and stable, which can withstand multiple cycles of drying and rehydration, remaining intact in various buffers. Proteins covalently immobilized on the bilayer by the terminal p-nitrophenylcarbonyl or carboxyl group of PEG remain higher bioactivity than that immobilized on silicon surface directly. © 2009 Springer Berlin Heidelberg. |
关键词 | Bi-layer Carboxyl Groups Covalent Immobilization Functionalized Imaging Ellipsometry Microfluidic Array Phosphatidylethanolamine Phospholipids Bilayer Silicon Surfaces |
WOS记录号 | WOS:000299998500551 |
URL | 查看原文 |
收录类别 | CPCI-S ; EI |
语种 | 英语 |
引用统计 | |
文献类型 | 会议论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/60310 |
专题 | 力学所知识产出(1956-2008) |
推荐引用方式 GB/T 7714 | Chen YY,Wang ZH,Liu Y,et al. Silicon surface modification with supported phospholipids bilayer for biosensor based on imaging ellipsometry[C]IFMBE Proceedings,2008. |
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