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Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor
Huang, Chenghong; Yang, QingLin; Song, Fayi; Chen, Nan; Liao, Xiaoling; Yao, Bo; Zhang, ShiTong; Chen, Yanyan; Jin G(靳刚); Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
Source PublicationINTEGRATED FERROELECTRICS
2016
Volume171Issue:1Pages:70-78
ISSN1058-4587
AbstractCarbohydrate side chain in antibody Fc region can be oxidized into aldehyde group by sodium m-periodate, which can simultaneously couple with the amino-group on silicon surface obtained by chemistry modification. This combination will introduce C=N linkage and site-directed immobilization of antibody, which was confirmed by AFM observation. The acquired antibody layer can then be used for immunoassay for alpha-fetoprotein (AFP). The combined antigen is directly detected by imaging ellipsometry in grayscale format. The proposed method was further compared with commercial kit by determination 20 clinic samples with significant correlation (R = 0.93).
KeywordAlpha-fetoprotein Imaging Ellipsometry Immunoassay
DOI10.1080/10584587.2016.1172004
Indexed BySCI ; EI
Language英语
WOS IDWOS:000378406600010
WOS KeywordATOMIC-FORCE MICROSCOPY ; IMAGING ELLIPSOMETRY ; VISUALIZATION
WOS Research AreaEngineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter
Funding OrganizationThis work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032, cstc2014jcyjA10029).
DepartmentNML纳米生物光学
ClassificationQ4
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/71955
Collection国家微重力实验室
Corresponding AuthorHuang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
Recommended Citation
GB/T 7714
Huang, Chenghong,Yang, QingLin,Song, Fayi,et al. Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor[J]. INTEGRATED FERROELECTRICS,2016,171(1):70-78.
APA Huang, Chenghong.,Yang, QingLin.,Song, Fayi.,Chen, Nan.,Liao, Xiaoling.,...&Jin, G .(2016).Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor.INTEGRATED FERROELECTRICS,171(1),70-78.
MLA Huang, Chenghong,et al."Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor".INTEGRATED FERROELECTRICS 171.1(2016):70-78.
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