Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor

doi:10.1080/10584587.2016.1172004

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	Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor
	Huang, Chenghong; Yang, QingLin; Song, Fayi; Chen, Nan; Liao, Xiaoling; Yao, Bo; Zhang, ShiTong; Chen, Yanyan; Jin G(靳刚); Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
发表期刊	INTEGRATED FERROELECTRICS
	2016
卷号	171 期号:1 页码:70-78
ISSN	1058-4587
摘要	Carbohydrate side chain in antibody Fc region can be oxidized into aldehyde group by sodium m-periodate, which can simultaneously couple with the amino-group on silicon surface obtained by chemistry modification. This combination will introduce C=N linkage and site-directed immobilization of antibody, which was confirmed by AFM observation. The acquired antibody layer can then be used for immunoassay for alpha-fetoprotein (AFP). The combined antigen is directly detected by imaging ellipsometry in grayscale format. The proposed method was further compared with commercial kit by determination 20 clinic samples with significant correlation (R = 0.93).
关键词	Alpha-fetoprotein Imaging Ellipsometry Immunoassay
DOI	10.1080/10584587.2016.1172004
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000378406600010
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; IMAGING ELLIPSOMETRY ; VISUALIZATION
WOS研究方向	Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter
项目资助者	This work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032, cstc2014jcyjA10029).
课题组名称	NML纳米生物光学
论文分区	Q4
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/71955
专题	微重力重点实验室
通讯作者	Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
推荐引用方式 GB/T 7714	Huang, Chenghong,Yang, QingLin,Song, Fayi,et al. Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor[J]. INTEGRATED FERROELECTRICS,2016,171,1,:70-78.
APA	Huang, Chenghong.,Yang, QingLin.,Song, Fayi.,Chen, Nan.,Liao, Xiaoling.,...&Jin, G .(2016).Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor.INTEGRATED FERROELECTRICS,171(1),70-78.
MLA	Huang, Chenghong,et al."Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor".INTEGRATED FERROELECTRICS 171.1(2016):70-78.

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