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Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin
Li YK; Liu W; Jin G(靳刚); Niu Y(牛宇); Chen YP; Xie MX
2018-07-03
发表期刊ANALYTICAL CHEMISTRY
卷号90期号:13页码:8002-8010
ISSN0003-2700
摘要

Analysis of trace low molecular weight (LMW) proteins in serum using the label-free imaging ellipsometry (IE) immunosensor is still a challenge due to the lack of an effective signal amplification strategy and the serious nonspecific adsorption. Herein we have developed a sandwich strategy mediated IE (SSIE) immunosensor to enable the immunodetection of LMW protein biomarkers in serum samples. We have first found that the weak binding affinity and the insufficient surface amount density of the ligand are two important factors which hinder the detection of LMW proteins in serum using the IE immunosensor. Then we have deduced that the sandwich strategy can amplify the detection signal of IE and avoid the nonspecific adsorption in serum. As a validation of the serological detection of LMW proteins, the SSIE immunosensor has been used to accomplish the quantitative detection of procalcitonin (PCT) in serum. Compared with other PCT analysis methodologies, the SSIE immunosensor enjoys the advantages of simplicity, rapidity, and sufficient sensitivity. Furthermore, we have proposed the criteria to predict the ability of the SSIE immunosensor for the detection of LMW protein biomarkers in serum, which can make the detection of LMW proteins smart and efficient.

DOI10.1021/acs.analchem.8b00888
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收录类别SCI ; EI
语种英语
WOS记录号WOS:000438008600031
关键词[WOS]Sepsis Diagnosis ; Tumor-markers ; Immunoassays ; Biosensor ; Adsorption ; Complex ; Sensor
WOS研究方向Chemistry, Analytical
WOS类目Chemistry
项目资助者International Science and Technology Cooperation Program of China [2015DFG32390] ; National Basic Research Program of China [2015CB352100] ; National Natural Science Foundation of China [11704218, 81472941, 21305147, 81671784] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017026]
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力学所作者排名1
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文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/77828
专题国家微重力实验室
通讯作者Niu Y(牛宇); Chen YP; Xie MX
作者单位1.Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China
2.Tsinghua Univ, Inst Microelect, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engineered Construct & Mechanobio, NML, Beijing 100190, Peoples R China
4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
5.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
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Li YK,Liu W,Jin G,et al. Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin[J]. ANALYTICAL CHEMISTRY,2018,90(13):8002-8010.
APA Li YK,Liu W,Jin G,Niu Y,Chen YP,&Xie MX.(2018).Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin.ANALYTICAL CHEMISTRY,90(13),8002-8010.
MLA Li YK,et al."Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin".ANALYTICAL CHEMISTRY 90.13(2018):8002-8010.
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