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Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin
Li YK; Liu W; Jin G(靳刚); Niu Y(牛宇); Chen YP; Xie MX
Source PublicationANALYTICAL CHEMISTRY
2018-07-03
Volume90Issue:13Pages:8002-8010
ISSN0003-2700
Abstract

Analysis of trace low molecular weight (LMW) proteins in serum using the label-free imaging ellipsometry (IE) immunosensor is still a challenge due to the lack of an effective signal amplification strategy and the serious nonspecific adsorption. Herein we have developed a sandwich strategy mediated IE (SSIE) immunosensor to enable the immunodetection of LMW protein biomarkers in serum samples. We have first found that the weak binding affinity and the insufficient surface amount density of the ligand are two important factors which hinder the detection of LMW proteins in serum using the IE immunosensor. Then we have deduced that the sandwich strategy can amplify the detection signal of IE and avoid the nonspecific adsorption in serum. As a validation of the serological detection of LMW proteins, the SSIE immunosensor has been used to accomplish the quantitative detection of procalcitonin (PCT) in serum. Compared with other PCT analysis methodologies, the SSIE immunosensor enjoys the advantages of simplicity, rapidity, and sufficient sensitivity. Furthermore, we have proposed the criteria to predict the ability of the SSIE immunosensor for the detection of LMW protein biomarkers in serum, which can make the detection of LMW proteins smart and efficient.

DOI10.1021/acs.analchem.8b00888
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000438008600031
WOS KeywordSepsis Diagnosis ; Tumor-markers ; Immunoassays ; Biosensor ; Adsorption ; Complex ; Sensor
WOS Research AreaChemistry, Analytical
WOS SubjectChemistry
Funding OrganizationInternational Science and Technology Cooperation Program of China [2015DFG32390] ; National Basic Research Program of China [2015CB352100] ; National Natural Science Foundation of China [11704218, 81472941, 21305147, 81671784] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017026]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/77828
Collection国家微重力实验室
Corresponding AuthorNiu Y(牛宇); Chen YP; Xie MX
Affiliation1.Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China
2.Tsinghua Univ, Inst Microelect, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engineered Construct & Mechanobio, NML, Beijing 100190, Peoples R China
4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
5.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li YK,Liu W,Jin G,et al. Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin[J]. ANALYTICAL CHEMISTRY,2018,90(13):8002-8010.
APA Li YK,Liu W,Jin G,Niu Y,Chen YP,&Xie MX.(2018).Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin.ANALYTICAL CHEMISTRY,90(13),8002-8010.
MLA Li YK,et al."Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin".ANALYTICAL CHEMISTRY 90.13(2018):8002-8010.
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