Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium

doi:10.1152/ajpcell.00083.2017

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	Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium
	Tong CF(佟春芳); Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Gong YX(龚一心); Yang H ; Feng SL ; Du Y; Huang DD ; Long M(龙勉)
发表期刊	AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
	2018-09-01
卷号	315 期号:3 页码:C409-C421
ISSN	0363-6143
摘要	Flowing polymorphonuclear neutrophils (PMNs) are forced to recruit toward inflamed tissue and adhere to vascular endothelial cells, which is primarily mediated by the binding of beta(2)-integrins to ICAM-1. This process is distinct among different organs such as liver and brain; however, the underlying kinetic and mechanical mechanisms regulating tissue-specific recruitment of PMNs remain unclear. Here, binding kinetics measurement showed that ICAM-1 on murine hepatic sinusoidal endothelial cells (LSECs) bound to lymphocyte function-associated antigen-1 (LFA-1) with higher on-and off-rates but lower effective affinity compared with macrophage-1 antigen (Mac-1), whereas ICAM-1 on cerebral endothelial cells (BMECs or bEnd. 3 cells) bound to LFA-1 with higher on-rates, similar off-rates, and higher effective affinity compared with Mac-1. Physiologically, free crawling tests of PMN onto LSEC, BMEC, or bEnd. 3 monolayers were consistent with those kinetics differences between two beta(2)-integrins interacting with hepatic sinusoid or cerebral endothelium. Numerical calculations and Monte Carlo simulations validated tissue-specific contributions of beta(2)-integrin-ICAM-1 kinetics to PMN crawling on hepatic sinusoid or cerebral endothelium. Thus, this work first quantified the biophysical regulation of PMN adhesion in hepatic sinusoids compared with cerebral endothelium.
关键词	brain ICAM-1 integrin kinetics liver
DOI	10.1152/ajpcell.00083.2017
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000444021100014
关键词[WOS]	BLOOD-BRAIN-BARRIER ; 2-DIMENSIONAL KINETICS ; NEUTROPHIL RECRUITMENT ; LEUKOCYTE RECRUITMENT ; LIVER METASTASIS ; INFLAMMATION ; SELECTIN ; MOTILITY ; MICROPIPETTE ; MECHANICS
WOS研究方向	Cell Biology ; Physiology
WOS类目	Cell Biology ; Physiology
项目资助者	National Natural Science Foundation of China [31230027, 91642203, 31661143044] ; Chinese Academy of Sciences Strategic Priority Research Program [XDB22040101] ; Frontier Science Key Project [QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601]
论文分区	二类
力学所作者排名	1
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/77952
专题	微重力重点实验室
作者单位	1.[Tong, Chun-Fang 2.Zhang, Yan 3.Lu, Shou-Qin 4.Li, Ning 5.Gong, Yi-Xin 6.Yang, Hao 7.Feng, Shi-Liang 8.Du, Yu 9.Huang, Dan-Dan 10.Chinese Acad Sci, Natl Micrograv Lab, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing, Peoples R China 11.[Tong, Chun-Fang 12.Zhang, Yan 13.Lu, Shou-Qin 14.Li, Ning 15.Gong, Yi-Xin 16.Yang, Hao 17.Feng, Shi-Liang 18.Du, Yu 19.Huang, Dan-Dan 20.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing, Peoples R China 21.[Zhang, Yan 22.Lu, Shou-Qin 23.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tong CF,Zhang Y,Lv SQ,et al. Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2018,315,3,:C409-C421.
APA	佟春芳.,章燕.,吕守芹.,李宁.,龚一心.,...&龙勉.(2018).Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,315(3),C409-C421.
MLA	佟春芳,et al."Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 315.3(2018):C409-C421.

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