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Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium
Tong CF(佟春芳); Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Gong YX(龚一心); Yang H; Feng SL; Du Y; Huang DD; Long M(龙勉)
Source PublicationAMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
2018-09-01
Volume315Issue:3Pages:C409-C421
ISSN0363-6143
AbstractFlowing polymorphonuclear neutrophils (PMNs) are forced to recruit toward inflamed tissue and adhere to vascular endothelial cells, which is primarily mediated by the binding of beta(2)-integrins to ICAM-1. This process is distinct among different organs such as liver and brain; however, the underlying kinetic and mechanical mechanisms regulating tissue-specific recruitment of PMNs remain unclear. Here, binding kinetics measurement showed that ICAM-1 on murine hepatic sinusoidal endothelial cells (LSECs) bound to lymphocyte function-associated antigen-1 (LFA-1) with higher on-and off-rates but lower effective affinity compared with macrophage-1 antigen (Mac-1), whereas ICAM-1 on cerebral endothelial cells (BMECs or bEnd. 3 cells) bound to LFA-1 with higher on-rates, similar off-rates, and higher effective affinity compared with Mac-1. Physiologically, free crawling tests of PMN onto LSEC, BMEC, or bEnd. 3 monolayers were consistent with those kinetics differences between two beta(2)-integrins interacting with hepatic sinusoid or cerebral endothelium. Numerical calculations and Monte Carlo simulations validated tissue-specific contributions of beta(2)-integrin-ICAM-1 kinetics to PMN crawling on hepatic sinusoid or cerebral endothelium. Thus, this work first quantified the biophysical regulation of PMN adhesion in hepatic sinusoids compared with cerebral endothelium.
Keywordbrain ICAM-1 integrin kinetics liver
DOI10.1152/ajpcell.00083.2017
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000444021100014
WOS KeywordBLOOD-BRAIN-BARRIER ; 2-DIMENSIONAL KINETICS ; NEUTROPHIL RECRUITMENT ; LEUKOCYTE RECRUITMENT ; LIVER METASTASIS ; INFLAMMATION ; SELECTIN ; MOTILITY ; MICROPIPETTE ; MECHANICS
WOS Research AreaCell Biology ; Physiology
WOS SubjectCell Biology ; Physiology
Funding OrganizationNational Natural Science Foundation of China [31230027, 91642203, 31661143044] ; Chinese Academy of Sciences Strategic Priority Research Program [XDB22040101] ; Frontier Science Key Project [QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601]
Classification二类
Ranking1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/77952
Collection国家微重力实验室
Affiliation1.[Tong, Chun-Fang
2.Zhang, Yan
3.Lu, Shou-Qin
4.Li, Ning
5.Gong, Yi-Xin
6.Yang, Hao
7.Feng, Shi-Liang
8.Du, Yu
9.Huang, Dan-Dan
10.Chinese Acad Sci, Natl Micrograv Lab, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing, Peoples R China
11.[Tong, Chun-Fang
12.Zhang, Yan
13.Lu, Shou-Qin
14.Li, Ning
15.Gong, Yi-Xin
16.Yang, Hao
17.Feng, Shi-Liang
18.Du, Yu
19.Huang, Dan-Dan
20.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing, Peoples R China
21.[Zhang, Yan
22.Lu, Shou-Qin
23.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Tong CF,Zhang Y,Lv SQ,et al. Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2018,315(3):C409-C421.
APA 佟春芳.,章燕.,吕守芹.,李宁.,龚一心.,...&龙勉.(2018).Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,315(3),C409-C421.
MLA 佟春芳,et al."Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 315.3(2018):C409-C421.
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