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The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment
Yu Y(于泳); Li K(李凯); Lin H(林海); Li JC(李吉成)
Source PublicationCRYSTALS
2018-11-01
Volume8Issue:11Pages:Ar-400
ISSN2073-4352
Abstract

Space is expected to be a convection-free, quiescent environment for the production of large-size and high-quality protein crystals. However, the mechanisms by which the diffusion environment in space improves the quality of the protein crystals are not fully understood. The interior of a microfluidic device can be used to simulate a microgravity environment to investigate the protein crystallization mechanism that occurs in space. In the present study, lysozyme crystals were grown in a prototype microchannel device with a height of 50 mu m in a glass-polydimethylsiloxane (PDMS)-glass sandwich structure. Comparative experiments were also conducted in a sample pool with a height of 2 mm under the same growth conditions. We compared the crystal morphologies and growth rates of the grown crystals in the two sample pools. The experimental results showed that at very low initial supersaturation, the morphology and growth rates of lysozyme crystals under the simulated microgravity conditions is similar to that on Earth. With increasing initial supersaturation, a convection-free, quiescent environment is better for lysozyme crystal growth. When the initial supersaturation exceeded a threshold, the growth of the lysozyme crystal surface under the simulated microgravity conditions never completely transform from isotropic to anisotropic. The experimental results showed that the convection may have a dual effect on the crystal morphology. Convection can increase the roughness of the crystal surface and promote the transformation of the crystal form from circular to tetragonal during the crystallization process.

KeywordProtein Crystallization Simulated Microgravity Microchannel
DOI10.3390/cryst8110400
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000451156400004
WOS KeywordCrystal-growth ; Macromolecular Crystallization ; Microfluidic Device ; Lysozyme Crystals ; Magnetic-field ; Nucleation ; Dynamics ; Facility ; Impurity ; Quality
WOS Research AreaCrystallography ; Materials Science, Multidisciplinary
WOS SubjectCrystallography ; Materials Science
Funding OrganizationNational Nature Science Foundation of China [11472282, 11672311]
Classification二类
Ranking1
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/78165
Collection国家微重力实验室
Recommended Citation
GB/T 7714
Yu Y,Li K,Lin H,et al. The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment[J]. CRYSTALS,2018,8(11):Ar-400.
APA Yu Y,Li K,Lin H,&Li JC.(2018).The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment.CRYSTALS,8(11),Ar-400.
MLA Yu Y,et al."The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment".CRYSTALS 8.11(2018):Ar-400.
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