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The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment
Yu Y(于泳); Li K(李凯); Lin H(林海); Li JC(李吉成)
发表期刊CRYSTALS
2018-11-01
卷号8期号:11页码:Ar-400
ISSN2073-4352
摘要

Space is expected to be a convection-free, quiescent environment for the production of large-size and high-quality protein crystals. However, the mechanisms by which the diffusion environment in space improves the quality of the protein crystals are not fully understood. The interior of a microfluidic device can be used to simulate a microgravity environment to investigate the protein crystallization mechanism that occurs in space. In the present study, lysozyme crystals were grown in a prototype microchannel device with a height of 50 mu m in a glass-polydimethylsiloxane (PDMS)-glass sandwich structure. Comparative experiments were also conducted in a sample pool with a height of 2 mm under the same growth conditions. We compared the crystal morphologies and growth rates of the grown crystals in the two sample pools. The experimental results showed that at very low initial supersaturation, the morphology and growth rates of lysozyme crystals under the simulated microgravity conditions is similar to that on Earth. With increasing initial supersaturation, a convection-free, quiescent environment is better for lysozyme crystal growth. When the initial supersaturation exceeded a threshold, the growth of the lysozyme crystal surface under the simulated microgravity conditions never completely transform from isotropic to anisotropic. The experimental results showed that the convection may have a dual effect on the crystal morphology. Convection can increase the roughness of the crystal surface and promote the transformation of the crystal form from circular to tetragonal during the crystallization process.

关键词Protein Crystallization Simulated Microgravity Microchannel
DOI10.3390/cryst8110400
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000451156400004
关键词[WOS]Crystal-growth ; Macromolecular Crystallization ; Microfluidic Device ; Lysozyme Crystals ; Magnetic-field ; Nucleation ; Dynamics ; Facility ; Impurity ; Quality
WOS研究方向Crystallography ; Materials Science, Multidisciplinary
WOS类目Crystallography ; Materials Science
项目资助者National Nature Science Foundation of China [11472282, 11672311]
论文分区二类
力学所作者排名1
引用统计
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/78165
专题国家微重力实验室
推荐引用方式
GB/T 7714
Yu Y,Li K,Lin H,et al. The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment[J]. CRYSTALS,2018,8(11):Ar-400.
APA Yu Y,Li K,Lin H,&Li JC.(2018).The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment.CRYSTALS,8(11),Ar-400.
MLA Yu Y,et al."The Study of the Mechanism of Protein Crystallization in Space by Using Microchannel to Simulate Microgravity Environment".CRYSTALS 8.11(2018):Ar-400.
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