| Cell senescence alters responses of porcine trabecular meshwork cells to shear stress | |
Du, Ruotian1; Li, Dongyan1; Zhu, Meng1; Zheng, Lisha1; Ren, Keli2; Han, Dong2 ; Li L(李龙)3,4; Ji, Jing1; Fan, Yubo1
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| 通讯作者 | Li, Long(lilong@lnm.imech.ac.cn) ; Ji, Jing(09714@buaa.edu.cn) ; Fan, Yubo(yubofan@buaa.edu.cn) |
| 发表期刊 | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
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| 2022-11-21 | |
| 卷号 | 10页码:14 |
| ISSN | 2296-634X |
| 摘要 | Mechanical microenvironment and cellular senescence of trabecular meshwork cells (TMCs) are suspected to play a vital role in primary open-angle glaucoma pathogenesis. However, central questions remain about the effect of shear stress on TMCs and how aging affects this process. We have investigated the effect of shear stress on the biomechanical properties and extracellular matrix regulation of normal and senescent TMCs. We found a more significant promotion of Fctin formation, a more obvious realignment of F-actin fibers, and a more remarkable increase in the stiffness of normal cells in response to the shear stress, in comparison with that of senescent cells. Further, as compared to normal cells, senescent cells show a reduced extracellular matrix turnover after shear stress stimulation, which might be attributed to the different phosphorylation levels of the extracellular signal-regulated kinase. Our results suggest that TMCs are able to sense and respond to the shear stress and cellular senescence undermines the mechanobiological response, which may lead to progressive failure of cellular TM function with age. |
| 关键词 | shear stress senescence trabecular meshwork cell glaucoma mechanobiology |
| DOI | 10.3389/fcell.2022.1083130 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000894046900001 |
| 关键词[WOS] | SIGNAL-REGULATED KINASE ; OPEN-ANGLE GLAUCOMA ; ENDOTHELIAL-CELLS ; OXIDATIVE STRESS ; MATRIX METALLOPROTEINASES ; OUTFLOW FACILITY ; AQUEOUS OUTFLOW ; GENE-EXPRESSION ; LAMINA-CRIBROSA ; STIFFNESS |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| WOS类目 | Cell Biology ; Developmental Biology |
| 资助项目 | National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; [12232019] ; [U20A20390] ; [11827803] ; [11902327] ; [12272388] |
| 项目资助者 | National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS |
| 论文分区 | 一类 |
| 力学所作者排名 | 1 |
| RpAuthor | Li, Long ; Ji, Jing ; Fan, Yubo |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/91211 |
| 专题 | 非线性力学国家重点实验室 |
| 作者单位 | 1.Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn, Key Lab Biomech Mechanobiol,Minist Educ, Beijing, Peoples R China; 2.Natl Ctr Nanosci & Technol, Lab Biol Imaging & Nanomed, Beijing, Peoples R China; 3.Chinese Acad Sci, State Key Lab Nonlinear Mech, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Ruotian,Li, Dongyan,Zhu, Meng,et al. Cell senescence alters responses of porcine trabecular meshwork cells to shear stress[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2022,10:14. |
| APA | Du, Ruotian.,Li, Dongyan.,Zhu, Meng.,Zheng, Lisha.,Ren, Keli.,...&Fan, Yubo.(2022).Cell senescence alters responses of porcine trabecular meshwork cells to shear stress.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,14. |
| MLA | Du, Ruotian,et al."Cell senescence alters responses of porcine trabecular meshwork cells to shear stress".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10(2022):14. |
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