Calcium response in osteocytic networks under steady and oscillatory fluid flow

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	Calcium response in osteocytic networks under steady and oscillatory fluid flow
	Lu XL; Huo B(霍波); Park M; Guo XE; Guo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA.
发表期刊	BONE
	2012-09-01
卷号	51 期号:3 页码:466-473
ISSN	8756-3282
摘要	The fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca2+](i)) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteocyte-like cells using micro-patterning techniques to simulate the in vivo orderly organization of osteocyte networks. Sinusoidal oscillating fluid flow or unidirectional steady flow was applied on the cell surface with 2 Pa peak shear stress. It was found that the osteocytic networks were significantly more responsive to steady flow than to oscillatory flow. The osteocytes can release more calcium peaks with higher magnitudes at a faster speed under steady flow stimulation. The [Ca2+](i) signaling transients under the steady and oscillatory flows have significantly different spatiotemporal characters, but a similar responsive percentage of cells. Further signaling pathway studies using inhibitors showed that endoplasmic reticulum (ER) calcium store, extracellular calcium source, ATP, PGE(2) and NO related pathways play similar roles in the [Ca2+](i) signaling of osteocytes under either steady or oscillating flow. The spatiotemporal characteristics of [Ca2+](i) transients under oscillating fluid flow are affected more profoundly by pharmacological treatments than under the steady flow. Our findings support the hypothesis that the [Ca2+](i) responses of osteocytic networks are significantly dependent on the profiles of fluid flow.
关键词	Osteocyte Mechanotransduction Calcium Response Fluid Flow Steady Oscillatory Nitric-oxide Synthase Bone Cell Mechanotransduction Functional Gap-junctions Osteoblastic Cells Shear-stress Mc3t3-e1 Osteoblasts Molecular Regulation Mlo-y4 Osteocytes Mechanical Strain Atp Release
学科领域	生物力学
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000307617400019
项目资助者	NIH grants R21 AR052417, R01 AR052461, and RC1 AR058453 (XEG).
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	二类
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/46578
专题	微重力重点实验室
通讯作者	Guo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA.
推荐引用方式 GB/T 7714	Lu XL,Huo B,Park M,et al. Calcium response in osteocytic networks under steady and oscillatory fluid flow[J]. BONE,2012,51,3,:466-473.
APA	Lu XL,霍波,Park M,Guo XE,Guo, XE,&Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA..(2012).Calcium response in osteocytic networks under steady and oscillatory fluid flow.BONE,51(3),466-473.
MLA	Lu XL,et al."Calcium response in osteocytic networks under steady and oscillatory fluid flow".BONE 51.3(2012):466-473.

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