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Calcium response in osteocytic networks under steady and oscillatory fluid flow
Lu XL; Huo B(霍波); Park M; Guo XE; Guo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA.
Source PublicationBONE
2012-09-01
Volume51Issue:3Pages:466-473
ISSN8756-3282
AbstractThe fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca2+](i)) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteocyte-like cells using micro-patterning techniques to simulate the in vivo orderly organization of osteocyte networks. Sinusoidal oscillating fluid flow or unidirectional steady flow was applied on the cell surface with 2 Pa peak shear stress. It was found that the osteocytic networks were significantly more responsive to steady flow than to oscillatory flow. The osteocytes can release more calcium peaks with higher magnitudes at a faster speed under steady flow stimulation. The [Ca2+](i) signaling transients under the steady and oscillatory flows have significantly different spatiotemporal characters, but a similar responsive percentage of cells. Further signaling pathway studies using inhibitors showed that endoplasmic reticulum (ER) calcium store, extracellular calcium source, ATP, PGE(2) and NO related pathways play similar roles in the [Ca2+](i) signaling of osteocytes under either steady or oscillating flow. The spatiotemporal characteristics of [Ca2+](i) transients under oscillating fluid flow are affected more profoundly by pharmacological treatments than under the steady flow. Our findings support the hypothesis that the [Ca2+](i) responses of osteocytic networks are significantly dependent on the profiles of fluid flow.
KeywordOsteocyte Mechanotransduction Calcium Response Fluid Flow Steady Oscillatory Nitric-oxide Synthase Bone Cell Mechanotransduction Functional Gap-junctions Osteoblastic Cells Shear-stress Mc3t3-e1 Osteoblasts Molecular Regulation Mlo-y4 Osteocytes Mechanical Strain Atp Release
Subject Area生物力学
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Indexed BySCI
Language英语
WOS IDWOS:000307617400019
Funding OrganizationNIH grants R21 AR052417, R01 AR052461, and RC1 AR058453 (XEG).
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/46578
Collection国家微重力实验室
Corresponding AuthorGuo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA.
Recommended Citation
GB/T 7714
Lu XL,Huo B,Park M,et al. Calcium response in osteocytic networks under steady and oscillatory fluid flow[J]. BONE,2012,51(3):466-473.
APA Lu XL,霍波,Park M,Guo XE,Guo, XE,&Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA..(2012).Calcium response in osteocytic networks under steady and oscillatory fluid flow.BONE,51(3),466-473.
MLA Lu XL,et al."Calcium response in osteocytic networks under steady and oscillatory fluid flow".BONE 51.3(2012):466-473.
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