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Selectivity of biopolymer membranes using HepG2 cells
Lv DY(吕东媛); Gao YX(高宇欣); Luo CH(罗春花); Lv SQ(吕守芹); Wang Q(汪迁); Xu XH(许向红); Sun SJ(孙树津); Wang CZ(王成之); Long M(龙勉)
Source PublicationRegenerative Biomaterials
2015-02-10
Volume2Issue:1Pages:21-29
AbstractBioartificial liver (BAL) system has emerged as an alternative treatment to bridge acute liver failure to either liver transplantation or liver regeneration. One of the main reasons that the efficacy of the current BAL systems was not convincing in clinical trials is attributed to the lack of friendly interface between the membrane and the hepatocytes in liver bioreactor, the core unit of BAL system. Here, we systematically compared the biological responses of hepatosarcoma HepG2 cells seeded on eight, commercially available biocompatible membranes made of acetyl cellulose–nitrocellulose mixed cellulose (CA–NC), acetyl cellulose (CA), nylon (JN), polypropylene (PP), nitrocellulose (NC), polyvinylidene fluoride (PVDF), polycarbonate (PC) and polytetrafluoroethylene (PTFE). Physicochemical analysis and mechanical tests indicated that CA, JN and PP membranes yield high adhesivity and reasonable compressive and/or tensile features with friendly surface topography for cell seeding. Cells prefer to adhere on CA, JN, PP or PTFE membranes with high proliferation rate in spheriod-like shape. Actin, albumin and cytokeratin 18 expressions are favorable for cells on CA or PP membrane, whereas protein filtration is consistent among all the eight membranes. These results further the understandings of cell growth, morphology and spreading, as well as protein filtration on distinct membranes in designing a liver bioreactor.
KeywordBiocompatible Membrane Hepg2 Bioartificial Liver
DOI10.1093/rb/rbu018
URL查看原文
Language英语
Funding OrganizationThis work was supported by Strategic Priority Research Program of Chinese Academy of Sciences (grant XDA01030604) ; National High Technology Research and Development Program of China (grant 2011AA020109) ; National Natural Science Foundation of China (grants 31110103918 and 31470907) ; National Key Basic Research Foundation of China (grant 2011CB710904).
DepartmentNML分子-细胞生物力学与空间生命科学
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60698
Collection国家微重力实验室
Affiliation中国科学院微重力重点实验室
Recommended Citation
GB/T 7714
Lv DY,Gao YX,Luo CH,et al. Selectivity of biopolymer membranes using HepG2 cells[J]. Regenerative Biomaterials,2015,2(1):21-29.
APA Lv DY.,Gao YX.,Luo CH.,Lv SQ.,Wang Q.,...&Long M.(2015).Selectivity of biopolymer membranes using HepG2 cells.Regenerative Biomaterials,2(1),21-29.
MLA Lv DY,et al."Selectivity of biopolymer membranes using HepG2 cells".Regenerative Biomaterials 2.1(2015):21-29.
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