IMECH-IR  > 非线性力学国家重点实验室
Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement
Zheng X(郑旭); Shi F; Silber-Li ZH(李战华)
发表期刊MICROFLUIDICS AND NANOFLUIDICS
2018-11-01
卷号22期号:11页码:Ar-127
ISSN1613-4982
摘要Total internal reflection fluorescence microscopy (TIRFM) based on the evanescent wave provides a powerful imaging tool to measure nanoparticle dynamics near the interfaces in nanofluidic and biophysical systems. The most dramatic advantage of this technique is encoding the nanoparticle motion in the vertical dimension into intensity variation. However, large measurement uncertainty was found in previous works, which has been a major obstacle for applying TIRFM to nanofluidic studies. In this study, we investigate the statistical intensity distribution (SID) of the fluorescent nanoparticles in the evanescent field. We establish a theoretical description of the SID by considering the Boltzmann distribution of the nanoparticle concentration, the statistical particle size variation, and the focal plane thickness of the objective. The theoretical results show excellent agreement with the experimental SID histogram. We then develop a method to precisely determine the base intensity I-0 and decode the vertical positions of nanoparticles, by which the measurement uncertainty of TIRFM can be significantly reduced. This SID method is verified by the nanoparticle tracking velocimetry measurement near the wall using nanoparticles with diameters of 100nm or 250nm as tracers. Furthermore, our experiments show that the SID of nanoparticles is sensitive to the salt concentration of the solution and the nanoparticle properties, implying the possibility of using SID as a nano-probe to explore solution or colloidal properties in nanofluidics.
关键词TIRFM Nanoparticles Statistical intensity distribution (SID) NanoPIV PTV
DOI10.1007/s10404-018-2145-2
URL查看原文
收录类别SCI ; EI
语种英语
WOS记录号WOS:000448836500001
关键词[WOS]COLLOIDAL FORCES ; SLIP LENGTH ; VELOCIMETRY ; MICROCHANNEL ; MICROSCOPY ; PROFILES ; FIELD ; FLOW
WOS研究方向Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas
WOS类目Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
项目资助者National Natural Science Foundation of China [11572335, 11672358] ; CAS Key Research Program of Frontier Sciences [QYZDB-SSW-JSC036] ; CAS Strategic Priority Research Program [XDB22040403]
论文分区二类
力学所作者排名1
引用统计
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/78155
专题非线性力学国家重点实验室
作者单位1.[Zheng, Xu
2.Shi, Fei
3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Zheng X,Shi F,Silber-Li ZH. Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement[J]. MICROFLUIDICS AND NANOFLUIDICS,2018,22(11):Ar-127.
APA 郑旭,Shi F,&Silber-Li ZH.(2018).Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement.MICROFLUIDICS AND NANOFLUIDICS,22(11),Ar-127.
MLA 郑旭,et al."Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement".MICROFLUIDICS AND NANOFLUIDICS 22.11(2018):Ar-127.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[郑旭]的文章
[Shi F]的文章
[Silber-Li ZH(李战华)]的文章
百度学术
百度学术中相似的文章
[郑旭]的文章
[Shi F]的文章
[Silber-Li ZH(李战华)]的文章
必应学术
必应学术中相似的文章
[郑旭]的文章
[Shi F]的文章
[Silber-Li ZH(李战华)]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。