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Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement
Zheng X(郑旭); Shi F; Silber-Li ZH(李战华)
Source PublicationMICROFLUIDICS AND NANOFLUIDICS
2018-11-01
Volume22Issue:11Pages:Ar-127
ISSN1613-4982
AbstractTotal internal reflection fluorescence microscopy (TIRFM) based on the evanescent wave provides a powerful imaging tool to measure nanoparticle dynamics near the interfaces in nanofluidic and biophysical systems. The most dramatic advantage of this technique is encoding the nanoparticle motion in the vertical dimension into intensity variation. However, large measurement uncertainty was found in previous works, which has been a major obstacle for applying TIRFM to nanofluidic studies. In this study, we investigate the statistical intensity distribution (SID) of the fluorescent nanoparticles in the evanescent field. We establish a theoretical description of the SID by considering the Boltzmann distribution of the nanoparticle concentration, the statistical particle size variation, and the focal plane thickness of the objective. The theoretical results show excellent agreement with the experimental SID histogram. We then develop a method to precisely determine the base intensity I-0 and decode the vertical positions of nanoparticles, by which the measurement uncertainty of TIRFM can be significantly reduced. This SID method is verified by the nanoparticle tracking velocimetry measurement near the wall using nanoparticles with diameters of 100nm or 250nm as tracers. Furthermore, our experiments show that the SID of nanoparticles is sensitive to the salt concentration of the solution and the nanoparticle properties, implying the possibility of using SID as a nano-probe to explore solution or colloidal properties in nanofluidics.
KeywordTIRFM Nanoparticles Statistical intensity distribution (SID) NanoPIV PTV
DOI10.1007/s10404-018-2145-2
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000448836500001
WOS KeywordCOLLOIDAL FORCES ; SLIP LENGTH ; VELOCIMETRY ; MICROCHANNEL ; MICROSCOPY ; PROFILES ; FIELD ; FLOW
WOS Research AreaNanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas
WOS SubjectScience & Technology - Other Topics ; Instruments & Instrumentation ; Physics
Funding OrganizationNational Natural Science Foundation of China [11572335, 11672358] ; CAS Key Research Program of Frontier Sciences [QYZDB-SSW-JSC036] ; CAS Strategic Priority Research Program [XDB22040403]
Classification二类
Ranking1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/78155
Collection非线性力学国家重点实验室
Affiliation1.[Zheng, Xu
2.Shi, Fei
3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zheng X,Shi F,Silber-Li ZH. Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement[J]. MICROFLUIDICS AND NANOFLUIDICS,2018,22(11):Ar-127.
APA 郑旭,Shi F,&Silber-Li ZH.(2018).Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement.MICROFLUIDICS AND NANOFLUIDICS,22(11),Ar-127.
MLA 郑旭,et al."Study on the statistical intensity distribution (SID) of fluorescent nanoparticles in TIRFM measurement".MICROFLUIDICS AND NANOFLUIDICS 22.11(2018):Ar-127.
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